We were dealing with bacteria that has the ability to be harmful and to avoid illness/sickness we need to thoroughly wash our hands and be careful around the bacteria. Another precaution to take was to not cough into or put any bacteria from our bodies into the agar plate. Those bacteria could be extremely dangerous and shouldn’t be cultivated. Observations Located on final page(s) Application Questions 1. The purpose of heating the agar plates in the autoclave prior to inoculating them with microorganisms was to make sure that the lab results were exact.
Pseudomonas Aeruginosa The purpose of this lab was to identify the unknown bacteria culture given by our respected instructor using various differential tests. The identification of this unknown culture was accomplished by separating and differentiating between possible bacteria based on specific biochemical characteristics. Each test was used in a way to help recognize those specifics and bring us one step closer to identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, lactose and glucose fermentation, citrate, indole, urease, motility, H2S, EMB and MAC. The tests performed on the unknown bacteria culture were all used to determine and identify the bacteria.
Most differential stains have a challenge step that follows staining with a primary dye. In the Gram stain the challenge step is a rinse with either ethanol or acetone (either may be used). This step dehydrates and tightens the cell wall of Gram positives (mainly peptidoglycan) such that the rinse does not enter the cell. Gram negatives have mainly a lipid cell wall (even though they do contain peptidoglycan) that allows the challenge rinse to penetrate the cell and rinse out the crystal violet-iodine complex rendering the Gram negative cell colourless. Thus, the Gram negative cells must be stained to be seen, and this is done with the counter stain.
This keeps condensation which forms in the lid from dropping onto and disrupting the bacteria growing surface. 2. When ready to use, let
If diagnosed early, botulism can be treated with an antitoxin. This antitoxin prevents the neurotoxin from circulating in the blood. Keywords: Botulism, types of botulism, symptoms Botulism Botulism is a very rare but serious neurological disease that is caused by the bacterium called Clostridium botulinum. It is a bacterium that occurs in the soil. (Chaudry, 2011, para.1) Botulism is a rare disease with four naturally occurring syndromes.
Bacterial infections can usually be treated with anti-biotics however some types of bacteria can form a protective spore which can make them more resistant to heat and chemicals. The requirements for optimum growth are is a temperature of 37c, water, food, time, oxygen/ no oxygen. Common illnesses caused by bacteria are Salmonella, tuberculosis, MRSA, bronchitis, ear infections and tonsillitis. Virus-tend to be smaller than bacteria and in order for the cells to reproduce they need to be in a living host. The common way of treating a virus is through immunization as anti-biotics will not be effective against viral infections.
Jewelry has small crevices that can hold microorganisms. Sweat, dead skin, and dirt can combine with the warm semi-moist skin and harbor under the jewelry and cause bacterial growth. The removal of jewelry and watches will enable thorough washing of hands of wrists. While removing jewelry look around the sink area and make sure there is adequate liquid soap and paper towels within reach. One important thing to remember when preparing to properly wash your hands is try to avoid contact with the sink with your hands or clothing.
Causes of Urinary Tract Infection Writers Name Name, Section No Instructor’s Name Date Urinary Tract Infections; Overview A urinary tract infection (UTI) is an infection involving the kidneys, ureters, bladder, or urethra. These are the structures that urine passes through before being eliminated from the body. The kidneys are a pair of small organs that lie on either side of the spine at about waist level. They have several important functions in the body, including removing waste and excess water from the blood and eliminating them as urine. These functions make them important in the regulation of blood pressure.
The roles of normal flora are to produce compounds which kill other bacteria. It also lowers the pH level in our body so that other bacteria can’t grow. It helps to produce vitamins we are otherwise unable to produce like vitamin B12. It aids in boosting our immune system and helps us to digest food. Gut flora is the normal bacteria that is found mainly in our intestines as well as our stomach.
| Practical Report | | | | Practical Report Introduction This specific experiment will demonstrate both the efficiency and the disinfecting capabilities of the various disinfectants used and if the experiment is followed correctly the results deemed should be noted that if the substance possess a chemical substances that inhibits the growth of the bacterial culture placed onto the agar (E.Coli) that inhibition zones will be created and we can tabulate and record the observation thus walking away with an understanding of the efficiency of the tested disinfectants used and their success rate. What is known about E.Coli is that it is normally found in food it is a Gram negative, rod shaped bacterium that can help the body break down and assists the body in digestion of food (Dowshen, 2009).Although certain strains of the E.Coli are pathogenic to humans such as the serotype O157:H7, this specific strain of E.Coli is known to cause food poising and can escalate and become life threatening (Nordqvist, 2011). Domestos is chlorine based bleaching agent (Directa, 2009) and is a common household cleaning substance that is used. Handy Andy is a common and popular general house hold cleaner. Dettol is trusted antiseptic and disinfectant and is widely used to fight unwanted bacteria (Thompson D. M.) It has a distinct and characteristic phenolic odour.