Vitamin C Experiment

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Experiment to find the concentration of Vitamin C Aim: The aim of this experiment is basically to determine how much vitamin C is there in 3 different types of orange juice by carrying out the experiment using the same concentration of 0.01% DCPIP throughout the experiment and known volumes of the orange juices. Background: Vitamin C plays an important role in the body. Vitamin C is a water-soluble vitamin and your body doesn't store it and neither is it produced in the body, it is obtained from food and fruit. Vitamin C is found in many fresh fruits like the oranges, lemons, limes, grapefruit, cantaloupes, mangoes, papayas, and their juices. You need vitamin C for the growth and repair of tissues in all parts of your body. It helps the body make collagen, an important protein in skin, cartilage, tendons, ligaments, and blood vessels. Vitamin C is essential for healing wounds, and for repairing and maintaining bones and teeth. Vitamin C is also an Antioxidant; it prevents too much oxidation from occurring in haemoglobin. Vitamin C also helps in homeostasis by helping to produce adrenaline. Furthermore it helps to form collagen; lack of Vitamin C can lead to Scurvy or symptoms of scurvy. It also helps to produce white blood cells in the body. These prove how important Vitamin C is in the body. Method: In this investigation same volume of 3 different types of orange juice are used, to find out which one has the most Vitamin C. This investigation involves using the organic indicator called Dichlorophenolindophenol which abbreviates to DCPIP. The indicator is a dark blue colour which turns colourless when a known amount of it is used neutralize ascorbic acid which is Vitamin C. The amount of juices used to turn the DCPIP colourless will be recorded and the vitamin C content will be determined by measuring the amount of a known vitamin C concentration

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