Use of Raman Spectroscopy in Identifying Body Fluids

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Abstract Both destructive and non-destructive techniques can be useful for determining the identity of body fluids at crime scenes. However, on site investigators would prefer techniques that are non-destructive to the fluid sample so that investigators can subsequently perform DNA analysis to confirm the identity of the fluid. Techniques that produce instant results, in addition to preserving the sample, are even more beneficial in forensic sciences. Specific techniques such as Raman spectroscopy and fluorescence spectroscopy are potential candidates. Here we will be discussing the potential advantages of using Raman spectroscopy and fluorescence spectroscopy, compared to the current destructive techniques being used in body fluid analysis. Introduction The ability to detect body fluids at a crime scene is very important in the field of forensic science. Body fluids give insight into the nature of the crime, as well as identify those present at the crime scene. Further DNA analysis of these fluids helps authorities identify the victim and assailant, and provides evidence that can be used to convict the perpetrator. The most common types of body fluids found at crime scenes are blood, semen, and saliva (Virkler and Lednev, June 2008). Other body fluids such as urine, sweat, and vaginal fluid may also be present at a crime scene and provide valuable information as well (Virkler and Lednev, December 2008). Two types of tests are used to analyze body fluids, presumptive and confirmatory. The investigator must conduct a presumptive test to determine what type of body fluid is present at the crime scene, and subsequent confirmation testing is required for the evidence to be admissible in court (Virkler and Lednev, December 2008). Each body fluid has specific structural components that are associated with that fluid. For instance, the major distinctive

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