Transformation in Genetic Engineering

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#11: Transformation in Genetic Engineering Introduction: Genetic engineering refers to a set of technologies that are being used to change the genetic makeup of cells and move genes across species boundaries to produce novel organisms. The techniques involve highly sophisticated manipulations of genetic material and other biologically important chemicals. Genes are the chemical blueprints that determine an organism's traits. Moving genes from one organism to another transfers those traits. Through genetic engineering, organisms are given new combinations of genes—and therefore new combinations of traits—that does not occur in nature and, indeed, cannot be developed by natural means. Such an artificial technology is radically different from traditional plant and animal breeding. Genetic modification is a common practice, in today’s modern world, happening naturally and artificially from laundry soap to salmon; today’s manufacturers are looking for any edge to win a market. We will attempt to use a commercially available plasmid, with two genes, one that is Ampicillin resistant, another that glows green, to show that the E. coli bacteria has been transformed by uptake of the genes in the plasmid. Both will be able to visually been seen, one by growth, the second by color change upon use of a UV Light. Hypothesis: We hypothesized that the following plate would have/not have growth of bacteria, and further if the bacteria grew it would/would not be green florescence. Plate 1- Luria Broth, Wild Type-We believed that the plate 1 would have growth of bacteria, but would not glow green florescent upon UV light being introduced. Plate 2- Luria Broth, pGreen transformed cells- We believed that plate 2 would have growth of bacteria, and would glow green florescent upon UV light being introduced. Plate 3- Luria Broth with Ampicillin, Wild Type- We
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