Effects of Volume on Enzyme Activity Biology 103 Second Spring Semester Introduction Enzymes are biological catalysts that catalysis biochemical reactions in living calls. The purpose of a catalyst is to decrease the activation energy required for a reaction to happen naturally. Enzymes increase the reaction rate by molecules by two hundred million times faster opposed to if there no enzyme present. During a catalyzed reaction, a substrate binds to an active site which in-turn forms an enzyme-substrate complex. This is where the reactions occurs.
Effect of Enzyme Activity on Temperature Aim: The aim of this experiment is to investigate how effectively the enzyme amylase breaks down starch at different temperatures Research Question: How does the increase in temperature affect the time taken for amylase to breakdown 2ml of starch? Purpose: Background Information: Enzymes are biological catalysts that comprise the largest and most highly specialized class of protein molecules. Enzymes act as catalysts to increase the rates of chemical reactions. A fundamental property of enzymes is their specificity. Various enzymes have unique shape and chemical composition that creates a site, called and active site.
The maximum activity of proteins and enzymes is also largely due to Calcium because the binding of Calcium ions is necessary for the activation of seven clotting agents in the coagulation cascade. A coagulation cascade is a series of sequences that stops bleeding through clot formations (Higdon, 2003). Magnesium amounts to about 25 grams inside the body, and is the fourth most common mineral inside the body (Dietary supplements, 2009). Magnesium helps the body to
They work best at room temperature around 36-40 C. Introduction For this experiment, I will be looking at how the change in temperature affects the rate of reaction. If the temperature is too high or too low in the water bath the enzymes active site will denature. The best temperature is 37 degrees because this is the natural body temperature which is also called the optimum temperature so the reaction will take place faster. Method * Firstly you have to label a test tube with the temperature that you will be investigating. * Secondly you have to add five drops of phenolphthalein the test tube which you are using.
In our 2nd, we examined the effect of warmer temperature. Our research question was, ‘does catalase denature in stages or all at once as temperature increases?’ Our hypothesis was: if temperature increases, then the catalase will denature in stages as the temperature increase, the catalase will slowly stop working. We followed the procedures in the lab manual (choinski and karatit 2014) with the following expetions: For experiment 1, we used 1.5% hydrogen peroxide concentration and experimented this concentration at 4*c, 24*c (room temp. ), 44*c, 52*c and 60*c. For experiment 2, we used 3% hydrogen peroxide concentration and experiment this concentration at 21*c (room temp. ), 35*c, 45*c, 55*c, & 60*c. Figure 1: Effect of temperature on catalase activity.
Dependent Variable: The time taken in seconds the enzyme to react at different temperatures. This is because the temperature of the enzyme is reacting at will either make the enzyme reacts faster or slower. The rate of digestion will be calculated by 1/t (sec-1) Controlled Variables: concentration of enzyme (3 ml lipase) X nextag shop; Lipase Concentrate-HP 90 UltraCaps - Integrative Therapeutics; $24 (http://www.nextag.com/enzyme-lipase/compare-html) concentration of substrate (3 ml cooking oil) concentration of inhibitor (1 ml bile salt) All of these are fixed so that it does not affect the reaction. Introduction: Proteins that are made up of long chains of amino acids are called enzymes. These enzymes act as catalysts in chemical reactions which decreases the activation required for the reaction and consequently
1 mark AND Extreme pH denatures the enzyme, altering the shape of the active site and preventing the enzyme and substrate forming a complex, thereby decreasing the rate of the reactions. This is seen in a decrease in the amount of oxygen being produced. 1 mark c. This pH will need to be read from the graph 1 mark AND Optimum pH 1 mark Question 2 a. Any 2 of the following The volume the pH solution The volume of peroxide The surface area of the liver cube The same size test tube 1 mark b. The pH 1 mark c. Treatment group refers to all groups which are being manipulated or varied during an experiment.
Reactivity of the Enzyme Lactase By: Jonathan Figueroa Jonathan Figueroa BIO 104 Enzyme II Lab March 5, 2013 Reaction of the Enzyme Lactase Abstract In this experiment we will determine what circumstances are needed for the enzyme lactase to undergo its reaction and which will inhibit the reaction. Our hypothesis was that the lactase enzyme will react best between pH levels 6.8 and 8. We set up the experiment so that 5 different pH levels including 2, 4, 6.8, 8 and 10 are tested 3 times and averaged. When the experiment was completed our results determined that the pH levels did affect the enzyme’s productivity and the pH levels that was the most active was pH 6.8. Also from pH 2 – pH 6.8 the absorbance did increase, but from pH 8 – 10 the absorbance decreased.
Enzyme Activity Lab By: Cheyenne Sherrill Lab partners: Brandon, Sade, and Jason Due date: November 27th 2012 Mr. Clark D1 honors Procedure: All the metabolic activity and processes within the cell require enzymes to occur. This includes synthesis and decomposition reactions. Enzymes are specialized proteins (organic catalyst) that speed up the rates of reactions that would otherwise be to slow or not able to occur at all in the cell. 1) Sand and peroxide- a pinch of sand and 2ml of hydrogen peroxide in a standardized test tube. Measure the height of the reaction with a ruler in millimeters.
Internal Assessment To test how temperature affects the rate of enzyme activity in the liver? Name: Chandre Putter Grade: 11 Words: 2380 Design Section Research Question/Aim How does temperature affect the rate of enzyme activity in the liver? Introduction Enzymes are ‘globular proteins that work as catalysts’ (1), meaning they speed up chemical reactions (metabolic reactions) without having to be altered. Enzymes are made by living cells and also speed up biochemical reactions. Living creatures produce thousands of enzymes, and the reason for this is because; ‘most enzymes only catalyze one biochemical reaction’ (1), and many different enzymes are need to do this.