3. I then measured out 750ml of tap water and added it to the pot where my cabbage was. 4. I then started boiling the cabbage while I assembled the 7 different substances including the coke and antacid as per the special instructions for this lab. 5.
At the bottom of the petri dishes we level it with four quadrants to make sure that each of the antibiotics were placed in the right quadrant and be able to see how the bacteria acted in respect to the placement of the antibiotic. One of the quadrants was kept as the control of the experiment. There were six antibiotic but each group obtain only three of the antibiotic for each bacteria. When placing the bacteria on the petri dish we used a pipette to obtain 100uL of the bacteria onto the petri dish. With a rod (spreader) that was dip in ethanol and then place under the busen burner in order to neutralize it; we spread the bacteria on every surface area of the petri dish.
Dip the strip in the milk, Wait five seconds, Record Data 6. Now add a few drops of each acid or base to each cup 7. Dip a new glucose a strip and record your data 8. Add a few drops of enzyme to each cup 9. Use a new glucose strip and record Data: Conclusion: According to my data, pH levels do, in fact, affect the results of Lactex.
The discoloration is measured in percent transmittance of light over 30minutes at 5 minute intervals. The change in dye color is the associated with cellular respiration activity, and will be used to record the cellular respiration rate in mitochondria isolated from pulverized lima beans (Phaseolus lunatus) and subsequent effects of different substrate concentration, pH, and metabolic inhibitors . If the difference of light percent transmission produced by (DPIP) can be recorded over time associated with the cellular respiration rate then the rate of cellular respiration of mitochondrion in varying substrate concentrations, pH , and metabolic inhibitor solutions can be tested. The results from these experiments can be generalized and applied to other organisms with similar reactions to such substances. Materials and Methods The procedures employed in this laboratory are described in Cell Physiology and Metabolism Lab
I added varying levels of substrate to the test tubes in each experiment. The amount of substrates were .5 grams, 1 gram, 2 grams, 4 grams, and 8 grams. The output of the experiment (the dependent variable) was the number of molecules of product formed per minute at (106). RESULTS Test Tube # | pH Level | Amount of Substrate | Number of Molecules of Product Formed per Minute (106) | Test Tube #1 | 3 | .5g | 19 | Test Tube #2 | 3 | 1.0g | 39 | Test Tube #3 | 3 | 2.0g | 82 | Test Tube #4 | 3 | 4.0g | 96 | Test Tube #5 | 3 | 8.0g | 96 | | | | | Test Tube #1 | 5 | .5g | 39 | Test Tube #2 | 5 | 1.0g | 81 | Test Tube #3 | 5 | 2.0g | 168 | Test Tube #4 | 5 | 4.0g | 198 | Test Tube #5 | 5 | 8.0g | 198 | | | | | Test Tube #1 | 7 | .5g | 72 | Test Tube #2 | 7 | 1.0g | 145 | Test Tube #3 | 7 | 2.0g | 300 | Test Tube #4 | 7 | 4.0g |
Experiment 11 Lab Assignment Answer Sheet: Chromatography of Food Dyes Name: Pre-Lab: Define the Rf value of a compound: It is the distance traveled by the compound divided by the distance traveled by the solvent front. Data and Observations: Record the distance travelled by the sample and the solvent in the table below and calculate the Rf values. Table 1. Color|Blue1|Blue2|Red3|Red40|Yellow5|Yellow6|Solvent| Distance(mm)|50mm|0mm|10mm|21mm|28mm|23mm|50mm| Rf|1|0|0.2|0.42|0.56|0.46|1| Table 2. Substance|Kool-Aid®: Grape|Kool-Aid®: Strawberry|Solvent| Distance (mm)| 19mm(red)|28mm(red)|48mm| Distance (mm)| 47mm(blue)||48mm| Rf|0.39 & 0.98|0.58|1| Table 3.
In this experiment the class took solid flakes of six different metals and tested the effect they had on the color of the flame they were placed in. This is done by taking six wooden splints which had previsly been soaked in water so they’d be able to pick up the chemical. Next, the laboratory burner was turned on and a flame was lit. Then, one of the splints was used to pick up a chemical and held in the fire to have it’s color recorded. This was six times total times, to test each chemical’s color.
Protects the bacteria from phagocytosis allowing the bacteria to stay in the body 6. pure culture 7. It is differential based on hemolysis of the agar. Hemolysis can be wide-narrow band beta, alpha, gamma, or none. 8. candle jar in microbiology is used for anaerobiosis in which a lit candle is placed in an air tight jar and if it went out, it would be because it used up all the available oxygen. 9. any streptococcus capable of hemolyzing erythrocytes, classified as α-hemolytic type, producing a zone of greenish discoloration much smaller than the clear zone produced by
Do not go into detail about the protocol. The purpose of the experiment was to determine the optimal pH and temperature of a phosphatase enzyme found in a crude protein extract isolated from alfalfa sprouts. The temperature optimum was found to be 55°C, and the pH optimum was found to be 5. Thus, the phosphatase enzyme can be classified as an acid phosphatase. 5.
Aim: To find out the effect of the temperature on the permeability of the cell membrane. Research Question: Effect of temperature on amount of pigment Background: Cell membranes contain many different types of molecules/substances that help maintain the overall structure, fluidity and functioning of the membrane. It is mainly consisted of a phospholipid bilayer, with a hydrophilic phosphate head and a hydrophobic lipid tail. The phospholipid bilayer is effective in stopping molecules from exiting or entering the call. Since the membrane has a non-polar layer in its centre and two polar layers on either side, it is difficult for both polar and non-polar molecules to pass through both layers.