It is a polysaccharide that functions as a carbohydrate store and is an important part of the human diet used as an energy source. It is a polymer of glucose sugar which means it is composed of many glucose molecules linked in a chain. Plants store the starch instead of simple sugars. Cellulose is an insoluble substance which is the main part of plant cell walls and vegetable fibres such as cotton. It is also a polysaccharide consisting of chains of glucose monomers.
The beaker was then moved to a stir plate with a stir bar and held in place by a ring stand. A funnel attached to an aspirator hose was fixed above to serve as a fume hood. With the aspirator and stir plate both on, 4.79 mL of 6 M HNO3 was added slowly to the beaker until the copper dissolved and the solution turned a light blue color. The hood could now be removed and 10.02 mL of distilled water was added to the solution. While stirring, 6 M NaOH was added drop-wise until the solution became basic, turning red litmus paper blue.
At the high pHs, the enzyme lactase is denatured and is no longer able to break down the sugar lactose in milk. Therefore, no glucose is present and the test strips remain blue. . The optimum range for the activity of lactase would be considered pH 2-7. The water control tube should match the tube with a
The solution with the precipitate was divided in to two tubes and centrifuged. The formed precipitate was left behind after washing the liquid out. Then we added another 5 mL and centrifuged again and washed the tube again. Eventually when got our Zinc Hydroxide in the test tubes. We then performed the two tests: Acid and base.
Then, the test media is then incubated at 37 ° C, for 18-24 hours. Rinsing reusable instruments The samples were rinsed with 40 ml of pyrogen-free water using a glass beaker that is free from pyrogens. Endotoxin testing using STV A total of 0,2mL from the water obtained from the rinsing was placed in the STV containing LAL reagent and was shaken for 20 to 30 seconds. Then STV was placed in an incubator at 37 ° C for 60 ± 2 minutes. STV was then observed by reversing the reaction tube in one smooth motion.
After a few minutes the acid dissolved the capsule creating a black foam that then turned into a clear liquid again. Once the liquid was clear again, the clamp was closed and the beaker was weighed. The temperature of the water was also taken. This was then repeated again for Trial 2 with a new capsule. Specialized Chemical Techniques: The specialized technique used in this experiment was
I tested for the presence of glucose by using Chemstrip 9. I tested for the presence of starch by using Lugol’s solution. A positive result for starch is a black-blue or bluish color and a negative result for starch is an amber color. In this experiment water moved into the dialysis bags by osmosis from an area of high concentration (surrounding liquid) into an area of low concentration (dialysis bag). This
One should be informed first about the importance of biomolecules before attempting any diet plan. Carbohydrates are very important for cellular function. Arguably, the most well known and important carbohydrate for the body is glucose. When complex sugars (polysaccharides) are consumed in the diet, they are eventually broken down to glucose, a monosaccharide. Glucose is then brought into the cytosol of cells where glycolysis (the breakdown of glucose) is initiated.
Digestive enzymes are hydrolytic enzymes. Their substances, or the molecules on which they act are organic food molecules which they breakdown by adding water to the molecular bonds, thus cleaving the bonds between the subunits or monomers. Digestive enzymes can function outside the body cells; their activity can be studied by test tubes (Marieb and Mitchell 2010). This experiment attempts to re-create the breakdown process that is normally done via digestion with Iodine as a vital component. It can be expected that once amylase reacts with the starch, maltose will then be broken down and less starch will be visible and more sugar will be apparent thus causing the solution mixed with iodine to become lighter and lighter.
Experiment 4: Study of Krebs Cycle using Mitochondria from Mung Bean Seedlings Matthew Foley 100769849 THURS PM BIOL 2200 A04 Bench #2 Introduction In this experiment the functional mitochondria were isolated from etiolated mung bean hypocotyls through the processes of homogenization and centrifugation. The process of homogenization disrupts the cell tissue in order to release the contents of the cells into the homogenate. Once the organelles have been released into the homogenate they are isolated by centrifugation. This process is effective due to the fact that different cellular structures move through the homogenate differently, based mostly on size and density. Therefore, different centrifugal forces and times are used, specific to which organelle or cellular component you are trying to isolate.