• After the flask was heated, I removed it from the heating mantel and allowed it to cool to room temperature. • Next, I poured 15mL of absolute ether and 9mL of bromobenzene into the 250mL flask and waited 5 minutes for any reaction. • There were no signs of reaction after 5 minutes; therefore the lab assistant used a glass stirring rod to crush the magnesium in order to get the reaction started. When this was done, the liquid became cloudy, rapidly bubbling, and the color turned rusty red. • Next, I added 25mL of absolute ether and attached a reflux condenser to the flask.
Put the plate in the Hot Air Oven at 100℃ for 30 minutes. After drying, the weight of biosurfactant was calculated with the help of following formula- Dry wt. of biosurfactant = (wt. of plate after drying – wt. of empty plate) Preliminary characterization of biosurfactant It was done by TLC method that is Thin Layer Chromatography method.
The reflux apparatus was place in the aluminum block. The block had been heated around 15 minutes which was to 120-130°C As the mixture was warmed, the white solid that formed was dissolved. The apparatus was lifted out of the aluminum block after a period of heating. Also, the flask was placed under the tap water to cool down the mixture to room temperature. Carefully 0.5mL increments of 3M sulfuric acid was
Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form. Then, centrifuge it for 1 minute. On the side, weight a boiling test tube containing 2 boiling chips. When the separation is complete, remove the small test tubes from the centrifuge and decant the supernatant into the boiling test tube. Add 1 mL of deionized water to the small test tube containing the precipitate and mix it and centrifuge it for 60 seconds.
“Analyzing and Synthesizing Potassium Aluminum Sulfate” Introduction: Potassium aluminum sulfate, also known as Alum, is a salt found in many commercially made products. Alum is an ingredient in commercially made baking powder, and gives baking powder the faint metallic taste. Alum is also used in the production of cucumbers, pickles, and maraschino cherries. The aluminum ions give the fruit a crisp taste because it strengthens the cell walls (2). Potassium aluminum sulfate is also used in some commercially produced and sold deodorants (3).
The last two cations should be separated with the observations of the additional NH4OH added. With that all the cations are separated, a confirmation test was given to all the cations. 20 drops of each cation solution were placed in new centrifuge tubes. A Bunsen burner was set up with a sharp blue cone that was inside of the flame, then a Nichrome loop was dipped into HCl then dipped into one of the cation
Allow the crystals to dry for 1 week then, weigh it, take a MP, and calculate the % yield. * Assemble the apparatus for reflux using the diagram (see figure 1), place boiling chips or a stirring bar in the bottom of the flask. * Weigh 2grams of finely ground nutmeg and combine with 10ml of diethyl ether in a 50ml round bottom flask. * Place a heating mantle under the round bottom, turned on and the heat was slowly adjusted until the mixture starts to boil for 45minutes, then let cool to room temperature by sitting it on the lab bench. * Pour a little ether over the nutmeg residue on the filter paper so that any Diethyl ethanol traces clinging to it is washed down and mixed with the filtered liquid underneath.
Dehydration of 2-Methylcyclohexanol Abstract The goal of this lab was to dehydrate 2-Methylcyclohexanol to obtain a cyclohexene using a concentrated acid. The percent yield for the final product was 69.6% and both saturation tests were positive for the presence of a double bond. The IR spectroscopy produced peaks between 1400 and 1600 cm-1, which suggests the presence of C=C double bonds. Introduction In this lab an alcohol is dehydrated using concentrated phosphoric acid, and sulfuric acid as a catalyst. The acid protonates the hydroxyl group, which creates water, a good leaving group.
Silver Nitrate can be used to remove warts by being used as a cauterizing agent in certain procedures. Dentists heal ulcers in the mouth with the help of this substance. To give a clear and detailed reflection, mirrors are coated with Silver Nitrate on one side. Silver Nitrate can also be used in photography, explosives, inks, and pottery. Reactant B- Sodium Chromate(aq)~Na2CrO4 Sodium Chromate is an odorless yellow crystalline powder.
I. Title: Extraction of Chlorophyll & Carotene from Spinach II. Objective: The objective of this experiment was to purify and extract chlorophyll & carotene from spinach by way of chromatography III. Procedure | Observations | 1. Obtain 0.5 grams of spinach leaves and use mortar along with 1 mL of acetone to grind the leaves and turn into a bright green color paste.