Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form. Then, centrifuge it for 1 minute. On the side, weight a boiling test tube containing 2 boiling chips. When the separation is complete, remove the small test tubes from the centrifuge and decant the supernatant into the boiling test tube. Add 1 mL of deionized water to the small test tube containing the precipitate and mix it and centrifuge it for 60 seconds.
Review Sheet Week 8 Exercise 21.5 Questions 1. Morphology and culturing. Pneumococci are Gram-positive, oval to lancet- shaped cocci that usually occur in pairs or short chains Morphology refers to an organism's structure, form and appearance. Gram stains allow for better viewing of bacterial cell structure 2. Alpha hemolysis 3.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Nurses must use sterile dressings on open skin surfaces to prevent infection. We must also keep patient equipment and supplies clean in order to prevent the spread of germs. Nurses must also use personal protective equipment like a mask, eye protection and a face shields if you are near a patient care activity that may involve a splash or spray of body fluids, they should then dispose of all single use personal protective equipment immediately after use. Cleaners should clean toilets with disinfectant to kills any germs. They should also clean any surfaces e.g.
After a series of washes to remove any impurities, the compound was dried and decanted, cholesterol was synthesized. Melting points were obtained from commercial cholesterol, dibromocholesterol, and synthesized cholesterol using the Fisher-John Melting Point Apparatus. A TLC plate was completed, spotting commercial cholesterol, dibromocholesterol, and synthesized cholesterol. The silica gel was used as the stationary phase and the ethyl acetate: hexane (30:70) was used as the mobile phase. The silver nitrate test was conducted to test for the functional group of 2°and 3° alkyl halides as positive and negative controls were used.
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).
5mL of acidified water will be measured, using a graduated cylinder, and will be transferred to the R tube, and will be immediately vigrously mixed with the reactants. Once the solution turns to an orange or red-brown color, a pipet will be used to quickly remove 30 drops of the solution, then transferred to the C tube, and the mixing will resume until the solution is close to room temperature. The solution will be filtered into the P tube, and the solution that is left in the R tube should be washed three times with 1mL of acidified water each time. The water should then be poured into the P tube, leaving the solid in the R tube. Using a test tube holder, heat the R tube over the Bunsen burner, moving the tube in a circular motion until all the water has evaporated.
The purpose of the laboratory is to determine the number of waters of hydration in aluminum potassium sulfate as well as the purity of the Alum we produce. Experimental1: The procedure for this laboratory was followed closely from Professor Abrams’ lab manual. In Part 1 of this laboratory an aluminum soda can was cut apart and the paint and lacquer was rubbed off with steel wool. Next, the aluminum can was rinsed off with tap H2O and then cut into small pieces. Using the analytical balance 1.0537 g of the aluminum can was weighed out.
When working in a microbiology lab, it is important to contain spills and discard materials properly. Describe how a spill should be cleaned up safely in a microbiology lab. (3 pts) Alert people in immediate area. Put on protective equipment. Cover area with disinfectant soaked-paper towels or with dry disinfectant.
The reaction was monitored and removed from the heat once the effervescence ceased, and then was allowed to cool. Concentrated hydrochloric acid was added while heating and stirring the reaction for 15 minutes. The product was then allowed to cool and was vacuum filtered to a maroon color, while being washed with water, 6M hydrochloric acid, ethanol, and acetone. Then allow the product to dry for five minutes on the funnel. The resulting product was [Co(NH3)5Cl]Cl2 and yield was 4.453g (.017 mol, 84.8%) Distilled water (25mL) was added to concentrated ammonia (5mL) in a 125mL Erlenmeyer flask.