Abstract The objective of the south street seaweed experiment is to make a tincture of iodine. Iodine is used commercially as an antiseptic on cuts and scrapes of the skin. Conceptually, one of the active ingredients of the tincture, iodide, can be extracted from seaweed. By adding the seaweed to water and applying heat energy into the mixture, we were able to extract iodide. After adding Iodine salts, and filtering the mixture our next goal was to test for three important chemicals that must exist in our mixture for it to be a true iodine tincture; Iodine, Iodide Ion and the triiodide ion.
Seaweed At Southside Seaport Warm Up 1. Coffee beans are crushed into small pieces, water is added to it and the mixture is heated over a flame. What do you think would happen to the coffee beans as it interacts with the water? Explain your prediction. The crushed coffee grounds would dissolve with the water, forming a solution of water and coffee.
Topic 5: On the wild side 17. Describe how to investigate the effects of temperature on the development of organisms (eg seedling growth rate, brine shrimp hatch rates). Procedure You will need: • Brine shrimp egg cysts • 2g sea salt for each treatment • 100cm³ de-chlorinated water for each treatment • 40cm³ beakers (one for each temperature to be tested) • Water baths or incubators (one for each temperature to be investigated) • Stirring rod • Magnifying glass • Pair of forceps • Fine glass pipette • Bright light Experiment: 1. Decide on a range of temperatures from 5°C to 35°C to be tested. 2.
The column is now ready for the addition of the mixture to be separated. 2.) Separation of the Fluorene and 9-Fluorenone Mixture: Accurately weigh 0.1g of the 1:1 mixture of fluorene and 9-fluorenone mixture, place it in a small test tube, and dissolve it in 0.5mL of petroleum ether. Add a few drops of dichloromethane, just enough to effect solution. Transfer the solution to the top of the column with a Pasteur pipet.
By ways of decanting (using a class stirring rod to guide solution), pour the solution into the funnel until it is about half an inch from the top. The solution should be clear and colorless 8) Allow the flask to cool. While the flask and solution are cooling, wash the funnel and beaker with LOTS of tap water to remove all traces of KOH. Also throw away any carbon on the filter paper. 9) Once the solution is cool enough to touch, add 35mL of 3M H2SO4 (measured out in 50mL graduated cylinder) SLOWLY AND STIR CONSTANLY.
Now to begin, pour 50 mL of the sodium phosphate buffer solution with a pH of 6.84 into the 150 mL beaker. From here on out, the sodium phosphate buffer solution will be referred to as simply the buffer solution. Next, locate the indicator called bromothymol blue (0.04%) and add 20 drops to your 150 mL beaker. The solution should then appear green. Next, obtain a 5 mL serological pipet and thoroughly rinse it with the buffer solution, then discard the buffer solution into the 250 mL beaker.
Write down everything you notice. Remove test tube from water and allow it to cool for 2 minutes in a small beaker of tap water. Add about 7.0 mL of 1M hydrochloric acid to the tube. Mix chemicals very thoroughly with a stirring rod. Record how long it takes for change to happen.
First of all, a pharmaceutical product can be made from seaweed by heating seaweed in water. This causes the product to be extracted from the seaweed. After that, filtration is needed to extract the seaweed from the water. Finally, by evaporating the product can be extracted from the water. 2.
Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear. Briefly rinse with distilled water. Counter-stain with safranin (secondary stain) for 1 minute then rinse with distilled water and gently blot dry with a paper towel. Lastly observe slide using oil immersion with the 100x
Bring the contents of the distillation flask to a gentle reflux (i.e., vapors are condensing in the first ¼ to 1/3 of the vertical condenser and dripping constantly back into the flask below). Maintain this reflux for 10 minutes, then increase the heat in order to distill the liquid into the collection flask, then the column, and even the distillation head with aluminum foil (shiny side IN). Continue distillation of the cyclohexene product until the volume in the distillation flask is reduced to about 1 mL. Ask the instructor to assist you in removing the heat source and sand bath for the almost empty flask; use Ove Gloves and/or clamps to avoid burns. |