Review Sheet Week 8 Exercise 21.5 Questions 1. Morphology and culturing. Pneumococci are Gram-positive, oval to lancet- shaped cocci that usually occur in pairs or short chains Morphology refers to an organism's structure, form and appearance. Gram stains allow for better viewing of bacterial cell structure 2. Alpha hemolysis 3.
The sea urchins are used by Biologists to unlock the mysteries of human diseases such as cancer. When you compare humans to sea urchins, quite a few amino acid
What is the frequency of tetracycline resistant bacteria in the cattle farm cultures? We will answer the questions by finding out if each bacteria is unique to a certain farm or all they all similar. Methods We performed serial dilutions on tetracycline plates. This helped us determine the frequency of tetracycline resistant bacteria I the original bacterial population. We used Polymerase Chain Reaction to amplify samples of the bacterial plasmid DNA.
A2 Applied Science (Biology) Unit 7: Planning and carrying out a Scientific Investigation TASK 1: Research Outline___________________ TASK 1: Research Outline___________________ Introduction to the Unit …………………………………………………………………. Page 3 Purpose and Objective of the investigation ……………………………………. Page 3 TASK 2: Research Plan____________________ TASK 2: Research Plan____________________ Client Group ………………………………………………………………………………….. Page 4 Grant Chart ……………………………………………………………………………………. Page 5 Basic Research (TCP, Dettol and E.coli bacteria) ……………………………… Page 5-7 Preparing my experiment ………………………………………………………………..
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
Biology lab: Methods and Results February 14, 2012 Genus species Methods: In order to conduct our experiment we started off with two petri dishes, six antibiotics and two types of bacteria to run our experiment. The bacteria that were being used in this experiment were E. coli and S. Marscescens. The antibiotics that are being used to combat the bacteria are called Penicillin (1929), Streptomycin (1947), Erythromycin (1952), Ampicillin (1961), Gentamicin (1971), and Ciprofloxin (1987). In the two petrib dish, each is individually chosen to be for E. coli and for S. Marscensen. At the bottom of the petri dishes we level it with four quadrants to make sure that each of the antibiotics were placed in the right quadrant and be able to see how the bacteria acted in respect to the placement of the antibiotic.
Procedure: Before beginning this lab please take the time to review this hand out and compare it to the information you know about organic molecules. Then, develop a hypothesis using the If, and Then statement. Go to http://www.okc.cc.ok.us/biologylabs/ and click on the “Organic Molecules” link. Read the introduction, and then proceed with tests for carbohydrates, proteins, fats, and foods. Record and answer all questions AS YOU GO.
It shows blue and purple for gram positive and red for gram negative. Simple stain gives the same color to all bacteria and makes it hard to differentiate between them. 5. In what kind of clinical situation would a direct smear report from the laboratory be of urgent importance? Blood infections, indication of cancer, or severe
CAUSES AND SPREAD OF INFECTION 1. Understand the causes of infection 1.1 Identify the differences between bacteria, viruses, fungi and parasites Bacteria - Typically a few micrometres in length, bacteria have a number of shapes, ranging from spheres to rods and spirals. Bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep portions of Earth's crust. Bacteria also live in symbiotic and parasitic relationships with plants and animals.
placed in a spectrophotometer. The data collected was used to view the growth of the bacteria and can be used to compare the growth of this E. coli strain to other E. coli strain, bacteria and the general bacterial growth curve. The data collected can make a template to possibly classify unknown bacteria. Not all bacteria replicate and grow at the same rate. Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis.