Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter gap so that the sides of the paper are not touching. The paper was then put into a beaker of a hydrochloric acid, ethanol, and butanol mixture, being sure that the paper does not touch the sides of the beaker, and then topped.
APPARATUS: Burette (50 cmᶾ), Pipette (25 cmᶾ), two conical flasks (250 cmᶾ), two beakers (250 cmᶾ), funnel, wash bottle, retort stand, boss and clamp, evaporating dish, pipette filler, hot-plate. MATERIALS: Approximately 2.0 mol dmˉ³ hydrochloric acid, 1.0 mol dmˉ³ sodium hydroxide, methyl orange indicator. METHOD: 1. Firstly, the burette was rinsed with a little hydrochloric acid and filled up to just above the zero mark. 2.
Add the HCl SLOWLY to the magnesium by running it down the side of the beaker. Adding the acid too quickly will result in the spattering of the acid. Write down any observations of the reaction. 6. After all the acid has been completely added and the reaction has stopped, use a pipette to add a few extra drops of acid into the beaker until the reaction stops.
The watch glass was removed with the beaker tongs. Using a rubber bulb and a stirring rod to stir the solution continuously, 15.00mL of .25M BaCl2 solution was added to the solution in the beaker. The watch glass is replaced and the solution is keep hot but not boiling for 15 minutes. The precipitate was allowed to settle. When the liquid above the precipitate was clear, the solution was tested for completeness of precipitation when a few drops of BaCl2 solution were added from a pipette.
Dip the slide into Eosin solution for 7 times and remove the excessive solution with a paper towel. 3. Dip the slide into new methylene blue for 9 times and remove the excessive solution with a paper towel. 4. Use the incinerator to allow it to dry completely.
Once it is completely distilled, remove your filtered material and add 2 mL of dichloromethane. Swish the flask, and then place into a small beaker. Next, place the beaker with the distilled liquid on a heating mantle and heat to a gel like substance. Make sure not to burn it. The next processes that will be
A. barium sulphate B. calcium carbonate C. silver chloride D. sodium nitrate 3. An element X forms a hydroxide, which dissolves, both in acids and in alkalis. What could X be? A. aluminium B. calcium C. copper D. iron 4. In which reaction is dilute sulphuric acid not behaving as an acid?
(b) Calculate the volume of 0.2M UO3- needed to react with 20.00 cm3 of 0.1M Cr2O72-. 3. 24.40 g of hydrated iron(II) sulphate, FeSO4.xH2O was dissolved and made up to 1.0 dm3 of aqueous solution, acidified with sulphuric acid. 25.00 cm3 of the solution was titrated with 20.00 cm3 of 0.022M potassium manganate(VII) solution for complete oxidation. a) Write the equation for the reaction.
6- Place only the edge of the Q-tip at the top the Flame. 7- Remove it when you see the of light being given off to avoid burning the Q-tip. 8- Clean up procedure: Discard used Q-tips to the bin, cover back compounds and put them up in a safe place, pour away distilled water in the sink, disconnect the Bunsen burner and clean it if stained,clean the lab test surroundings with paper towel to ensure no stain is left, wash your hands remove your goggles only when all equipments have been placed in safe places. Compound | Flame Colour Observation | 1 LiNO3
Look in the water bath on your table for a flask labeled DMA. This flask contains Davis Minimal Agar that has been autoclaved to make it sterile, and is being kept at 47 C to keep it liquefied. 3. Think about these important points in pouring a petri plate before doing it: a) You must work quickly, because once the container of minimal agar is removed from the bath, it will start to harden within 2-3 minutes. b) When pouring agar into the petri dish, pour just enough to fill the dish about half way.