Make sure the rubber stopper is securely in place since gas can easily leak out. Collecting the gaseous product: Prepare the gas collection bottle by positioning the bottle so that its mouth is. directly over the hole at the bottom of the trough; this is where the gas will enter the bottle. After the reaction, slide a square of glass over the mouth of the bottle while the bottle is still under water. Keeping the glass fixed to the mouth, raise the collection bottle and invert.
Care must be taken when squeezing the pipet bulb on the filter pipet. Too much pressure might cause the filter to leak or fall off. Add about 2 mL of fresh tert-butyl methyl ether to the solid in the RB flask, warm briefly, let the solids settle for a minute, and pipet the liquid to the centrifuge tube as before. Again allow the solids to settle briefly in the centrifuge tube, then filter the liquid through the pressure filtration apparatus, into the same 25 mL Erlenmeyer flask. Doing a rinse such as this helps to ensure that any trimyristin that was left behind in the RB flask and centrifuge tube is not lost, thereby helping to ensure that
When an indicators color changes it shows the presence of an organic compound. The purpose of using distilled water as one of the substances is that it is the control so you can see the differences. Also, in the substances distilled water was mixed in with them. The control group in part C is distilled water. The purpose of washing the test tubes thoroughly is so the chemicals don’t cross contaminate and affect the outcome of another section of the lab.
Autoclaving is the most common method, it uses steam under pressure to sterilize instruments and is the most reliable method. When sterile or disinfected items are required in the care setting single use, disposable items are recommended. 2.2 Describe how and when cleaning agents are used Cleaning agents are substances which are used to remove dirt, dust, stains and bad smells from surfaces. The purpose of these in the care setting is to avoid the spread of dirt and contaminants. There are many different cleaning agents which will be used in different places and circumstances.
* Clean/disinfect spillages of blood/body fluids as per guidance. * Maintain a clean environment to minimise the accumulation bacteria potentially harmful to patients. * Immunisations and infection control * Principles of good hygiene * Importance of appropriate protective clothing * General cleanliness * Principles of isolation nursing * Immunisation and infection control * Safe practice procedures * Management of outbreaks of infection Another common practice is that when in health care workers look are looking at areas of a patient's body which are prone to spread infection or are vulnerable to infection they wear protective clothing. This includes face masks and disposable gloves. This is done for two main reasons firstly so the workers themselves don't get infected if the patient has an infection and
After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid. Place another dry test tube in a 50mL beaker and weigh it. Find a bottle of barium iodide and record the name and molar mass. Then, weight out either anhydrous barium iodide or barium iodide dehydrate into this test tube and dissolve is it in 2 mL of deionized water. Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form.
Using a test tube holder, heat the R tube over the Bunsen burner, moving the tube in a circular motion until all the water has evaporated. The P tube will be carefully heated next, for about 10 minutes, until all the water has evaporated, and there is a white substance in the tube. After the water has evaporated, continue heating the tube over the burner for a minute longer, being careful to watch out for purple gas and yellow spots. The P tube
Wait for the bubbling to subside between additions so that the reaction does not overflow the flask. 6. When all the acetic acid has been added, swirl flask or stir for two minutes with a glass stirring rod. 7. When the solution is completely calm, move the flask to a hot plate and heat it to boiling.
Add 3.0 mL of 1.0 M Copper (II) nitrate to the test tube. Add 5.0 mL of sodium hydroxide to the test tube; mix the reaction with a stirring rod. Feel the bottom of the tube and write down any changes you notice. Place test tube into the heated beaker and heat the tube until no changes are happening. Write down everything you notice.
Place a funnel on the top of the buret and slowly add 5g of dry alumina. When all of the alumina has been added, rinse the inside of the buret with additional petroleum ether to flush down any alumina that may be stuck to the sides. Add another 1-cm layer of dry clean sand on top of the alumina. Drain the solvent from the column until the solvent is at a level just above the top of the sand. The column is now ready for the addition of the mixture to be separated.