Experiment Chromatography Of Food Dyes Abstract This experiment is to determine the presence of a mixture in a substance. Chromatography is used to separate substance in a mixture or separating components from a mixture. Using chromatography with a solvent we are able to separate the different mixtures found in a substance. Chromatography can tell if a sample is pure or if it is made up of several different substances. Experiment & Observation I gathered together my items; distilled water, salt, ruler, scissors, stapler pencil, tape, 16 toothpicks, Kool-Aid drink mix strawberry and grape, set of McCormick food coloring red, yellow, green and blue, small bag of M&M candy, plastic beaker 50 mL, petri dish 60mm, well-plate 24, FDC blue dye #1 0.5 mL vial, FDC blue dye #2 0.5 mL vial FDC red dye #3 0.5 mL vial, FDC red dye #40 0.5 mL vial, FDC yellow dye #5 0.5 mL vial, FDC yellow dye #6 0.5 mL vial, unknown 0.5 mL vial, 3 filter paper chrom 14x7 cm.
Paper chromatography can be used in separating amino acids and anions, RNA fingerprinting, and testing histamines and antibiotics. (Infromation received from sonic.org Purpose The purpose of this experiment was to separate the dyes that these markers are composed of and show how chromatography works. Materials * Four different markers (including one black permanent marker) * Rubbing alcohol or isopropyl alcohol * Coffee filters (2) * Tall glasses or plastic cups (2) * Pencil * Ruler * Tape * Table salt * Water * Measuring cups/spoons * Clean pitcher or 2-liter bottle Procedure 1. Your first task is to cut the coffee filter into a rectangle measuring three cm by nine cm. You will need 2 for this lab.
* Leave to cool in the pan for 10-15 minutes then turn out onto a wire rack to cool completely. * Once the cake is cooled, slice it horizontally in half. Put the bottom slice onto your serving plate. * Drizzle 1 tbsp of Frangelico over the top of the cake on the plate. * To make the fudge frosting, place the chocolate pieces and butter in a heatproof bowl over a saucepan of barely simmering water.
Using the measuring cup, add two cup of confectioner’s sugar into the pitcher as well. If you have doubt about how sweet you like your Kool-Aid, start with one cup of sugar and add more as needed, tasting with each addition. Now, with your long-handled spoon submersed in the pitcher of water, carefully stir until all of the Kool-Aid powder dissolves. Then, with your spoon filled with Kool-Aid, do a final taste
Add color indicator 4. Obtain a burette and fill it with NaOH 5. Titrate the 10 mL with NaOH until it turns pink 6. Weigh a dry funnel with a filter paper and record the weight 7. Obtain an Erlenmeyer flask that has a vacuum opening and attach the vacuum tube to it 8.
Calculate the Normality of the vinegar using the previously given equation. Na = (Nb)(Volumeb) (Volumea) C. Calculate the mass of the acetic acid in grams using the previously given equation. Massa = (Na)(GMWa) D. Calculate the percentage of acetic acid using the previously given equation. % Acid = Massa(g/L) x 100 1000g/L Discussion and Conclusion: Questions: LabPaq question guidelines: Answer questions A and G in the lab manual. Skip questions B, C, D, E and F in the lab manual, and answer these instead: A.
Why? they both change color Experiment 2: Starch Test Fill in the table below with the results from the starch test experiment. Results Starch Test Solution Initial Color Color with Iodine Solution starch solution clear purple water clear yellow-green Iodine solution is added to a chocolate chip cookie solution. The color changes to purple. Based on this result, what biological molecules are present in the chocolate chip cookie solution?
Materials & Methods Materials: · Scale · 4 6” Dialysis Tubing · 4 Transfer Pipets · Sugar · Scissors · Rubber Bands · 4 Same-Sized Coffee Cups · 250ml Graduated Cylinder · Tape Measure · Sauce Pan · 3 600ml Containers · Plastic Covering · Spoon Methods: 1.) Place 4 6” pieces of dialysis tubing into coffee cups full of tap with and leave them for two hours prior to beginning the experiment. 2.) As you wait, prepare your three sugar solutions. For the first solution, pour 5 grams of sugar into 250ml graduated cylinder and add water up to the 250ml mark.
Why? Experiment 2: Starch Test Fill in the table below with the results from the starch test experiment. Results Starch Test Solution Initial Color Color with Iodine Solution starch solution water Iodine solution is added to a chocolate chip cookie solution. The color changes to purple. Based on this result, what biological molecules are present in the chocolate chip cookie solution?
Red velvet Cake Ingredients Cake: • 2 cups sugar • 1/2 pound (2 sticks) butter, at room temperature • 2 eggs • 2 tablespoons cocoa powder • 2 ounces red food coloring • 2 1/2 cups cake flour • 1 teaspoon salt • 1 cup buttermilk • 1 teaspoon vanilla extract • 1/2 teaspoon baking soda • 1 tablespoon vinegar • Icing: • 1 (8-ounce) package cream cheese • 1 stick butter, softened • 1 cup melted marshmallows • 1 (1-pound) box confectioners' sugar • 1 cup shredded coconut • 1 cup chopped pecans Directions Preheat oven to 350 degrees F. In a mixing bowl, cream the sugar and butter, beat until light and fluffy. Add the eggs one at a time and mix well after each addition. Mix cocoa and food coloring together and then add to sugar mixture; mix well. Sift together flour and salt. Add flour mixture to the creamed mixture alternately with buttermilk.