Once it is completely distilled, remove your filtered material and add 2 mL of dichloromethane. Swish the flask, and then place into a small beaker. Next, place the beaker with the distilled liquid on a heating mantle and heat to a gel like substance. Make sure not to burn it. The next processes that will be
Allow the mixture to cool for a few minutes then filter it, using either gravity or vacuum filtration. (We shall be using vacuum filtration.) Wash the residue in the funnel once with a little water and collect all the filtrate. 4. Pour all the filtrate and washings into a 250cm3 volumetric flask.
While the soil is soaking, add dispersing agent into the control cylinder (Sodium Hexametaphosphate 125ml) and fill it with water to the mark. 3. Shake the cylinder in such a way that the contents are mixed thoroughly. Insert the hydrometer and thermometer into the control cylinder and record temperature. 4.
Then by writing a balanced chemical equation and using the titration formula, Nb+Ma+Va=Na+MbVb , the molarity is able to be determined. Procedure: 1) Using the graduated cylinder add 10.0 mL of water into the Erlenmeyer flask. 2) Add 5.0 mL of HCl into the flask using another graduated cylinder because acid goes into water when mixing them. 3) Add three drops of phenolphthalein indicator into the flask. 4) Swirl the flask in circular movements to mix the substances.
Put the open end of the measuring cylinder upside down, filled with water, in the water 4. Put one end of the tube into the measuring cylinder 5. Put 20cm3 of hydrochloric acid into the boiling tube 6. Add the marble chips into the hydrochloric acid and put the other end of the tube with the rubber stopper, onto the boiling tube allowing all gas through the tube 7. Stir the solution by making a circle motion with the tube
The watch glass was removed with the beaker tongs. Using a rubber bulb and a stirring rod to stir the solution continuously, 15.00mL of .25M BaCl2 solution was added to the solution in the beaker. The watch glass is replaced and the solution is keep hot but not boiling for 15 minutes. The precipitate was allowed to settle. When the liquid above the precipitate was clear, the solution was tested for completeness of precipitation when a few drops of BaCl2 solution were added from a pipette.
10. Grasp the tube of sterile nutrient broth with free hand, remove the cap, and carefully flame the mouth of the tube. 11. Insert the inoculating loop into the sterile broth and move the loop back and forth in the tube to disperse the organism into the medium.
DNA Extraction Aim: To extract DNA from a carrot using house hold products. Method: * Step 1 – Pour 60 ml (¼ cup) of clear alcohol into a glass. Place the glass into the bowl of iced water to chill, then set aside. * Step 2 – Pour 120 ml (½ cup) of tap/distilled water into the measuring cup with the salt. Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved.
b) Open the cover of the petri dish halfway and pour in the agar to just cover the bottom of the dish. Try to minimize the introduction of bubbles. c) Repeat for all the dishes. d) Immediately rinse the flask with warm water to facilitate washing the flask. 5.
Experiment 1: Extraction of Caffeine from Tea The primary objective of the experiment is to separate the caffeine from tea leaves. The mixture of tea and the calcium carbonate is separated from tea leaves by vacuum filtration. The solvent used in extraction of caffeine is dichloromethane. Anhydrous magnesium sulfate is added to the dichloromethane solution to dehydrate the mixture. The dichloromethane is then separated from the mixture in the rotary evaporator.