Unknown Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 Due Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae isolated from the given #103 was a Gram-negative Rod. The purpose of this lab was to isolate and identify the genus and species of an unknown bacterium. It is important to identify an unknown microorganism because knowing how the bacteria work and how it is structured means knowing how it can affect humans. Unknown bacteria may also be used clinically many pharmaceutical drugs are based on products made by organisms (Katzung, B.G.2004). In order to identify the unknown organism a series of tests were performed.
After improved understanding of the causes of disease there was understanding that you could cure a disease. Behring used this and Koch’s work to isolate anti toxins that would otherwise ,harm the body, to fight Diphtheria, Behring then found a way to inject it. Paul Ehrlich ( a member of Koch’s team) used his team to build on this work , he knew that certain dyes stained specific microbes (Koch’s work) furthermore with Behring’s work Paul tres to find a cure for syphillis a “magic bullet” that would only target the microbes and not the body. He managed to research seven years which was only made possible because of government funds. In 1909 Dr Hata had joined the research team and he reviewed the previous experiments.
The silver nitrate test was conducted to test for the functional group of 2°and 3° alkyl halides as positive and negative controls were used. The silver nitrate test was conducted for a presence of the functional group alkenes and there were positive and negative control. The percent yield of dibromocholesterol collected was 91.98% and the percent yield of synthesized cholesterol collected was 140%. The percent recovery of the commercial cholesterol was 127%. This can only suggest there were some impurities in the synthesized cholesterol but other results prove it to be pure.
(Page 7 ch1) One contribution is the journal he has written is about the improved methods of scientific crime detection and this still reports today. The second contribution is his treatise written about scientific disciplines in the field of criminal investigations. (Page 8 ch 1) Karl Landsteiner figured out how to divide blood into groups and today there are four different groups A,B,AB,O. Then Leone Lattes takes this information and puts it to work with figuring out the types of blood with dried blood. (pg10 ch1) The first person to apply the principals of forensic science was Edmond Locard, he began in the attic of the Lyons police department.
6/28/2012 The 1st lab procedure performed was the gram stain. Previously, before starting the gram stain I prepared a bacterial smear on a glass slide and used the heat fixing method which was done to help the bacteria cells adhere to the glass, kill them, and also helps make the cells easier to stain by coagulating the cells proteins. The gram stain test helped me identify rather my bacterium would be a negative or positive and determined the microscopic morphology (shape, and arrangement) of my bacterium as well. The test
If the urea is broken down by H. pylori in the biopsy, there is a change in color around the biopsy on the slide. This means that there is an infection with H. pylori in the stomach. Biopsies also may be cultured in the bacteriology laboratory for the presence of H. pylori; however, this is done infrequently since other simpler tests are available. A recently-developed test for H. pylori is a test in which the presence of the bacterium can be diagnosed with a sample of stool. The test uses an antibody to H. pylori to determine if H. pylori is present in the stool.
Macleod, who provided the lab space and direction to Banting and Best, put his research team to work on the production and purification of insulin. J.B. Collip joined the team of reaserchers and with his technical knowledge the four men were able to purify insulin to use on diabetic patients. The first test were conducted on Leonard Thompson early in 1922. These were a grest success. word of this discovery spread rapidly around the world, giving hope to many diabectic people who were near death.
Ocean County College Department of Chemistry [Observations of Chemical Changes] Submitted by Andrew Grimm Date Submitted: 5/31/14 Date Performed: 5/31/14 Lab Section: Chem-181DL2 Course Instructor: Amal Bassa Purpose The main goal in this experiment is to observe the properties of simple chemical reactions, and relating their chemical properties to household products. Procedure To begin this experiment I placed two drops of NaHCO3 into well A1 of the 96-well plate, and then added two drops of HCl into well A1. Next I placed two drops of HCl into well B2, and added two drops of BTB, and observed the color. Then I placed two drops of NH3 into well C1 and added one drop of BTB, and observed the color. Next I added two drops of HCl into well D1, and then added two drops of blue dye, and recorded what I saw.
Radu Purtuc | Chemistry 231 - Lab | Section 32 Ronnie :: team-member TECH 705, SEPARATING ACIDS AND NEUTRAL COMPOUNDS BY SOLVENT EXTRACTION - MACROSCALE EXTRACTION July 6, 2006 Lab Report INTRODUCTION Purpose: using extraction technique to separate acetanilide, p-toluic acid, and p-tert-butylphenol; then measure the melting points of each compound and comparing them to the statistical values. Background: separating acids and neutral compounds by solvent extraction is a useful technique used in practice by chemists to separate organic compounds from mixture of other compounds. It generally involve several steps, with limiting factors being the number and output purity of compounds to be extracted from source mixture. A number of specific compound solvents not inter-miscible are used to separate and dissolve compounds, and a physical transfer between solvents is employed. An example of use is cleaning compounds of impurities, where different solvents work as a molecular filter that interacts differentially with compound and impurities respectively.
At the Albert Einstein College of Medicine, he received his MD degree in 1960. While working at SUNY Downstate Medical Center, he researched potassium and sodium in living cells. This guided him to the first experiments with nuclear magnetic resonance (NMR). By using nuclear magnetic resonance, the differences between normal tissue and tumors can be found to diagnose cancer. His first experiments were on rats where he found large differences in the strength and length of the NMR signals given off by the cancerous and healthy tissues.