The cells are then treated with a soap solution that degrades the Plasma Membrane and Nuclear Membrane, releasing the cell's contents. The soap also causes proteins and lipids to precipitate out of the solution. Salt is used to stabilize the DNA, which can then be isolated from the solution by adding a layer of ethanol, which causes the DNA to precipitate. Pre-Lab Questions 1. Why is the Banana treated with a soap solution?
Obtain an Erlenmeyer flask that has a vacuum opening and attach the vacuum tube to it 8. Insert funnel and rinse the funnel with distilled water 9. Turn vacuum on and pour the pink solution into the Erlenmeyer flask, the precipitate should stay on top of the filter 10. Rinse the beaker out until all residue is gone 11. Try to rinse the precipitate in the funnel until the pink color is gone 12.
The beaker was then moved to a stir plate with a stir bar and held in place by a ring stand. A funnel attached to an aspirator hose was fixed above to serve as a fume hood. With the aspirator and stir plate both on, 4.79 mL of 6 M HNO3 was added slowly to the beaker until the copper dissolved and the solution turned a light blue color. The hood could now be removed and 10.02 mL of distilled water was added to the solution. While stirring, 6 M NaOH was added drop-wise until the solution became basic, turning red litmus paper blue.
To get mass of food burnt, I subtracted the initial mass by the final mass. I then repeated these steps for the other two samples making sure to clean and dry the beaker each time. I got the heat energy gained by multiplying the ΔT by the mass of the water and 4.184 J/g°C. Then I calculated the heat energy per unit mass by taking my heat energy gained and dividing it by the mass of food burnt to get Joules/ g. To get the Cal of the sample I took the J/g and divided it by 4.184 and then divided that answer by 1,000 to get my Calorie. Observations: Marshmallow: I noticed that the marshmallow caught fire pretty quick.
The aspirator was turned to medium high, and then the copper was poured onto wetted filter paper. Using distilled water to remove all copper from the beaker. Once completely on filter paper 6mL of acetone was added to the copper to help dry it out. The filter paper was then removed and set down to dry completely. Once dry the filter paper was weighed with the copper on it and subtracted from the original weight to see the amount of copper left after
The cooked starch acts as the food, which the caterpillar would eat, and the alpha amylase, which is a digestive enzyme common in saliva, is stirred with it to simulate the chewing and mixing of food and saliva which constitutes the first step in the digestive system. Next two pieces of dialyses tubing were prepared by clamping one end of each piece of tubing shut with a clamp, and twisting the other end until it opens. For the purpose of the experiment, the tubing will represent the intestine of the caterpillar.Continuing on, a clean pipette was used to transfer four pipettes worth of the starch and alpha amylase solution into one of the pieces of tubing, while four pipettes of plain starch was added to the other in order to form a control. Next, two large beakers were filled two thirds of the way with distilled water. Four droppers worth of Lugol’s reagent was added two each beaker.
This will aid in cushioning the fall and weighting the bottom of the cone. 6) Place the box containing the egg into the cone and tape it into place. 7) Your egg cone carton is now ready to be dropped. Make sure that you drop the cone carton it with the pointy end down. Results: After an exhaustive research period, we came to the conclusion that our egg cone carton did not cushion the egg enough from a side impact.
A coin needs to be used to extract pigments from the spinach leaf cells. Place a small section of the leaf on the top of the pencil line. Then place the chromatography paper in the cylinder so that the pointed end is barely in the solvent, make sure not to get the pigment in the solvent. Mark the bottom of each pigment band. Then measure the distance of each pigment starting at the bottom of the pigment origin to the bottom of the separated pigment band.
Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated. During this procedure, the iodine extract was filtered and several tests, both standard procedure and home- Objective: The purpose of this experiment is to understand how a single substance can be isolated from a mixture, like seaweed and water. Also, learn how to utilize filtration and extraction to make a tincture for testing for Iodine, Iodide Ion, and Triiodide Ion. Procedure: 1. 6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water.
Warm up Activity 1. The coffee beans would begin to disintegrate. The beans become smaller, the flavor and color from the beans will fill the water. Essentially the water will run through the coffee beans and extract its properties 2. The same thing will happen to the dry strips of seaweed.