Zing Substances. For ionizing substances, such as NaCl , 1mosm is 1mmole times the number of ions formed when each molecule dissociates. One mmole of NaCl is 58 mg, but when it dissociates, it yields 1 mmole of Na+ (23mg) and 1mmole of Cl ( 35mg). Therefore , 58 mg of NaCl is 2 mOsm of NaCl is put into a beaker and distilled water added to make 1 liter, the osmolarity is 2mOsm/l. A) How many mosm solute will 1 gram of NaCl yield?
The carbonate anion test mixes 1 mL of test solution and drops of 6M HCl. The formation of bubbles shows the presence of carbonate (CO32-). The acetate anion test heats 2 mL of the test solution, 1 drop of 18M H2SO4, and 1 mL (100%) ethanol. A fruity smell, using the wafting technique, shows the presence of acetate
Use a new glucose strip and record Data: Conclusion: According to my data, pH levels do, in fact, affect the results of Lactex. I say this because in my experiment, pre-enzyme, my solutions were all negative except for the soda. I used the test strips to determine if they were negative or positive solutions. After I put in the enzyme, Lactex, my results changed. Vinegar, Antacid, and Soap all became positive solutions after I put in the enzyme.
Using a measuring cylinder, add 50cm3 of 1.0mol dm-3 sulphuric(VI) acid to the thyme extract in the conical flask. 8. Titrate the solution in the conical flask with the potassium manganate(VII) solution until a pale pink colour persists for 10 seconds. 9. Repeat the titration until there are two titres within 0.1cm3 of each other.
More specifically in these experiments, we tested if there were reducing sugars with the Benedicts reagent in the first experiment and if there were starches present with Lugol’s solution in the second experiment. Hypothesis: The solutions with the highest amount of sugar will turn a red-orange when mixed and heated with the Benedict’s reagent. Null Hypothesis: The solutions with the highest amount of sugar will not turn a red-orange when mixed and heated with the Benedict’s reagent. In the first experiment to test if there were reducing agents, we used Benedict’s reagent. When using Benedict’s reagent, monosaccharides can be identified.
By using TLC and IR spectrum, we will see the difference between benzophenone (starting material) and diphenylmethanol (final product). Experimental Procedure: Benzophenone (358mg, 2.0mmol) was dissolved in ethanol (5ml) in a 25ml round bottomed flask. The solid did not completely dissolve, so the flask was swirled with magnetic stirrer bar. In a small test tube, sodium borohydride (87mg, 2.2mmol) was dissolved in cold water (1.5ml). This solution was added drop wise to the stirred ethanolic solution of benzophenone at room temperature.
If the compound does not melted after 2 minutes put “no” in the table. Part B - Solubility in Ethanol 1. Obtain six test tubes and add 25ml of ethanol to each test tube. 2. Add a two-gram sample of each of the above compounds to each test tube.
The dichloromethane is then separated from the mixture in the rotary evaporator. Figure 1: The structure of caffeine is very similar to those of purine bases (adenine and guanine) in DNA, therefore caffeine is a good substance to practice on for dealing with nucleic acids. Procedure Approximately 10g of tealeaves, CaCO3 (4.8g, 0.048 mol) and deionised H2O (100mL) is added into a beaker and boiled for 15 minutes. The mixture is then cooled to 20˚C and is filtrated using a Buchner funnel (vacuum filtration). The extraction process is when a solvent, dichloromethane (15mL) is added to the filtrate in a separatory funnel; the mixture is gently swirled together 3 times, and stopcock is released in between to vent the funnel.
In part two we were to determine if the cell membrane allowed all chemical substances to pass in and out of a cell with equal ease, and to determine whether the plastic bag was semi-permeable or not. We placed a small, sealed plastic bag filled with a cloudy starch solution in a beaker of hot water mixed with IKI solution, which turned the water amber colored. When we came back after 15 minutes, the starch solution, which was sealed in a plastic bag, was purple, while the IKI/water was still amber colored. Why might all of this have occurred? Well, in part one, the plant cell was originally in its home environment of a hypotonic solution (water), which was doing nothing to modify the cell.
Nutmeg comes from an East Indian tree. The seeds are rich compounds known as triglycerides, which are made up of glycerol esterfied to three fatty acids. Five grams of nutmeg was weighed and mixed with 50ml of mthylene chloride in a 100ml round bottom flask. A ruflux apparatus was set up and the mixture was brought to a boil in a steam bath. The mixture was refluxed to avoid evaporation while it boiled for 30 minutes.