Observing Temperature on Tyrosinase Essay

598 Words3 Pages
Observing the Affects Temperature has on the Rate of a Tyrosinase Reaction Linda Zheng, David Tran; Section 003 Introduction: It is important for a Lab technician to know the effect that temperature has on the rate of enzymes in a reaction to determine the optimal temperature to make product from substrates. We would like to ask how certain temperatures affect the rate of the Tyrosinase reaction on the protein L-DOPA. We hypothesize that temperature is a major component of the productivity of the enzyme, Tyrosinase, and that we will be seeing a large fluctuation of Tyrosinase activity depending on the temperature change because extreme temperatures do now allow the enzymes to function properly and they operate best at their own optimal temperature in between the extremes. In order to test this hypothesis, we conducted an experiment by filling five different tubes with a combination of reaction buffer, L-DOPA, and Tyrosinase, and setting them in five different temperature of water and observing the results in a spectrophotometer. We predicted a bell curve shape of a graph representing the change in temperature and the absorbance where the extreme temperatures will show that enzymes are unable to function properly and at high temperatures but they will operate best at their own optimal temperatures in between the extremes. Materials and Methods: The materials used in this experiment were: six glass cuvettes (one blank sample and five temperature labeled samples), micropipettes from Gilson (200 and 1000ul), a test tube holder, a flat surface, three water baths, a vortex mixer, reaction buffer, L-DOPA Sigma (D9628), Tyrosinase Sigma (D3824), and a spectrophotometer from Thermo Scientific. A control cuvette was created to set the spectrometer containing water, reaction buffer, L-DOPA, and Tyrosinase. Five cuvettes were incubated at 0, 25, 32, 42, and 60 degrees.The

More about Observing Temperature on Tyrosinase Essay

Open Document