The visual analysis of the results of the experiment will also be provided in which my qualitative data will be presenting which includes my own evaluation and observations of the experimental results. Quantitative & Qualitative Analysis The tables below show the results obtained by the first experiment in which I tested the effectiveness of TCP on E.coli Bacteria in 2 different petri
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
The sea urchins are used by Biologists to unlock the mysteries of human diseases such as cancer. When you compare humans to sea urchins, quite a few amino acid
Included in the lesson would be the effects that plants and animals have in complementing each other within the saltwater ecosystem b. New vocabulary words will be given: salt marsh, tide, cordgrass, marsh hay, burrow, gills c. Images will be studied and described with the key elements in the images explained d. Team Study Rules will be explained at the end of the lesson and posted for all students to see Rationale: Providing a lesson with most of the elements needed will give the students a head-start on discussion with each other are how to go about finishing the group work that is provided. Giving the students key concepts, skills and processes will help to determine the study teams. 2. Select Teams /Team Study: Teams are selected and take on the task of learning together about Saltmarshes a.
As part of an undergraduate project, a student was attempting to construct a restriction map for the plasmid pUC23 using the restriction enzymes EcoRI and BamHI. After carrying out both single and double enzyme digest reactions and electrophoresing each reaction mix through an aragose gel, the picture below is obtained, showing the number of DNA fragments produced in each reaction, along with the sizes of each fragment. From this information, construct a restriction map of the pUC23 for enzymes EcoRI and BamHI. Digest Performed: Sizes of Fragments Obtained:
6/28/2012 The 1st lab procedure performed was the gram stain. Previously, before starting the gram stain I prepared a bacterial smear on a glass slide and used the heat fixing method which was done to help the bacteria cells adhere to the glass, kill them, and also helps make the cells easier to stain by coagulating the cells proteins. The gram stain test helped me identify rather my bacterium would be a negative or positive and determined the microscopic morphology (shape, and arrangement) of my bacterium as well. The test
placed in a spectrophotometer. The data collected was used to view the growth of the bacteria and can be used to compare the growth of this E. coli strain to other E. coli strain, bacteria and the general bacterial growth curve. The data collected can make a template to possibly classify unknown bacteria. Not all bacteria replicate and grow at the same rate. Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis.
A coverslip was applied and the preparation observed in the microscope. The same procedure was followed to observe Gonium sp. from the mixed pond culture. A mixed culture of yeast (Saccharomyces
The cooked starch acts as the food, which the caterpillar would eat, and the alpha amylase, which is a digestive enzyme common in saliva, is stirred with it to simulate the chewing and mixing of food and saliva which constitutes the first step in the digestive system. Next two pieces of dialyses tubing were prepared by clamping one end of each piece of tubing shut with a clamp, and twisting the other end until it opens. For the purpose of the experiment, the tubing will represent the intestine of the caterpillar.Continuing on, a clean pipette was used to transfer four pipettes worth of the starch and alpha amylase solution into one of the pieces of tubing, while four pipettes of plain starch was added to the other in order to form a control. Next, two large beakers were filled two thirds of the way with distilled water. Four droppers worth of Lugol’s reagent was added two each beaker.
I will investigate and determine the effects of substrate concentration and pH on the initial rate of an enzyme-catalyzed reaction. Procedure: First I am gonna adjust the pH levels in five different test tubes, and then I am gonna add certain amount of substrate to each of those different test tubes and record different reactions in each of the test tubes. I am going to repeat this step four more times for each weight of substrate.