Microscopic Observation Of Plant Cells, Animal Cel

596 Words3 Pages
Microscopic Observation of Plant cells, animal cells and animal specimen 1. If the eyepiece on a microscope has a magnification of 10x, what is the total magnification with a 10x objective? Total magnification = magnification for objective X magnification of eyepiece. Thus, The total magnification for the microscope is Eyepiece 10x times objective 10x = 100x. What is the total magnification with a 45x objective? The total magnification = eyepiece 10x times objective 45x = 450x. 2. A microscope gives a total magnification of 1500x, but the image is too blurry to be useful. What might be the problem with this microscope? It can be cause either due to dirty eyepiece or objective lance or poor resolution. Other reason can be if oil immersion wasn't performed. 3. Why is it important to center a specimen on low power before attempting on focus on it at high power? When you increase the magnification, you decrease the field of view. Usually, with parfocal microscopes, whatever is in the center of the original field of view is also visible at higher magnification, but if it was off to one side, you probably wouldn't see it at the higher magnification. 4. A student focus on a specimen at low power and carefully centers it before changing to high power. At high power, however, he doesn't see the part of the specimen he was interested in. What might be the problem? The object the student is looking at is not circular, and possibly one part (in which s/he might be interested) extends far to one side, thus out of the field of view at the higher magnification. Change the lighting, If there is too much light, things can appear bleached out. S/he could adjust the iris to restrict the light directed through the specimen. Another possibility is that even slicing it with the razor blade was not enough to make the individual cells visible because there are

More about Microscopic Observation Of Plant Cells, Animal Cel

Open Document