Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated. During this procedure, the iodine extract was filtered and several tests, both standard procedure and home- Objective: The purpose of this experiment is to understand how a single substance can be isolated from a mixture, like seaweed and water. Also, learn how to utilize filtration and extraction to make a tincture for testing for Iodine, Iodide Ion, and Triiodide Ion. Procedure: 1. 6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water.
02 April 15, 2014 #7 Enterobacteriaceae, Escherichia coli Staphylococcaceae, Staphylococcus epidermidis ABSTRACT The objective of the Unknown Report is to identify two unknown bacterium based on a series of tests learned in the microbiology laboratory. The tests performed on organism A were Fluid Thioglycollate, Gram Stain, MR/VP, SIM, Simon Citrate, and LB plate. The results for organism A were Facultative Anaerobe for aerotolerance, Gram negative, MR positive and VP negative, the SIM test results were positive for Indole productivity and growth radiating but negative for sulfur production, Simon Citrate negative, and there was no red growth on the LB plate. These tests lead to the discovery of the bacterium Escherichia coli which
Use your 4 markers to draw a different colored dot on each of the pencil marks on the paper strip. Allow the markers ink to dry, and then go back recolor each dot. 5. Prepare the saltwater mixture by mixing 1/8 teaspoon of salt and three cups of water in a clean 2-liter bottle. Stir or shake the solution until it is dissolved.
We now slowly pour the solution into a funnel with filter paper. The extract along with 2 pieces of Iodine are added to a new beaker and left for 10 minutes. Finally to determine if the lab was successful, three tests are conducted for Iodine, Iodide and triiodide. The objective is to produce a tincture of iodine by extracting iodide and other components from seaweed. Warm up Activity 1.
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
P5 First I collected four test tubes; in each test tube I purred the solution that has been labelled A, B, C, and D in the container. Then I added a few drops of Iodine solution to the four test tubes. Container A was starch, because the colour changed to blue black, which is test for starch. Starch iodine blue black I purred the three solutions into the sink. Then I purred the solution from the container B, C, and D in to the three
After donning the appropriate safety gear I began by placing 3 separate sets of 10 drops of distilled water into an unused well of the 24 well plate. I added the following chemicals into one of the three sets of distilled water creating three separate chemical mixtures: HCI, Ammonia, and Sodium Hydroxide. I mixed all thoroughly with a toothpick and then sucked the mixtures into separate pipets. These were placed into the 24 well plate for later use. Using the 96 well plate I combined various chemicals together to observe the chemical changes that were created.
6/28/2012 The 1st lab procedure performed was the gram stain. Previously, before starting the gram stain I prepared a bacterial smear on a glass slide and used the heat fixing method which was done to help the bacteria cells adhere to the glass, kill them, and also helps make the cells easier to stain by coagulating the cells proteins. The gram stain test helped me identify rather my bacterium would be a negative or positive and determined the microscopic morphology (shape, and arrangement) of my bacterium as well. The test
The visual analysis of the results of the experiment will also be provided in which my qualitative data will be presenting which includes my own evaluation and observations of the experimental results. Quantitative & Qualitative Analysis The tables below show the results obtained by the first experiment in which I tested the effectiveness of TCP on E.coli Bacteria in 2 different petri
placed in a spectrophotometer. The data collected was used to view the growth of the bacteria and can be used to compare the growth of this E. coli strain to other E. coli strain, bacteria and the general bacterial growth curve. The data collected can make a template to possibly classify unknown bacteria. Not all bacteria replicate and grow at the same rate. Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis.