Marker Enzyme Assay Essay

1590 WordsOct 1, 20147 Pages
Transect and Quadrant Sampling Introduction Ecological methods are used to obtain and sample different data, two of which, the capture-based method and the plot-based method, are relevant to quantify the abundance of different organisms in an area (SDSU, 1993). Capture-based method is the type of sampling used for mobile and elusive species wherein organisms are marked, captured, and released. Plot-based method makes use of ecological tools such as transects and quadrats wherein individuals are counted in a known area. The census of plant and animal populations is relevant to study the patterns of organism abundance and distribution (Melville & Welsh, n.d.). The plot-based method was primarily used for this experiment in which transects and quadrats were utilized. Transect is a line stretched over an area of study. It is marked at regular intervals and purchased commercially as measuring tapes or ropes. It can be used to sample a uniform area or any area wherein there is a transition in habitats and populations. A measuring tape is run in a straight line between two points. The species that touch the transect line is considered relevant for the data (Griffin, 2000). A quadrat, on the other hand, is a framed area. It is usually in the form of metal or wooden frames, typically 0.25m2, 0.5m2, or 1.0 m2. It is a series of squares of a set size, which is placed in a certain area. The species within those quadrats are identified and recorded. It is used to collect data at locations with a sizable area in order to determine the abundance and/or diversity of the organisms in the area (Cox and Philippoff, n.d.). Transect lines and quadrats were given to the four groups. Our group, particularly was assigned the 0.25 m2 quadrat to be placed at the end of a measured 2m interval while some groups were given the transect and the 1 m2 quadrat. We then hypothesized

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