Liver Enzyme Essay

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Liver Enzyme Lab Purpose: The purpose of this experiment is to determine the optimal temperature of Catalase. Procedure: Place a small amount of blended liver a baggie Heat water on a hotplate (or use ice if needed) to bring your water bath to your assigned temperature (measure with thermometer) Place baggie with liver into water, brining both to desired temperature. When ready, add 2 filter paper discs to the liver in the baggie and let it sit in the water for 3-4 minutes. Meanwhile, set up your apparatus, as follows, to measure O2 production (volume in mL) *Perform TWO trials When ready, mix the peroxide with the discs, measure the volume of the gas produced. Materials: -Thermometer -Filter paper discs -Inverted graduated cylinder -Hydrogen peroxide -Cafeteria tray -Rubber Stopper with tube -Gas trapping bottle -Blender -Liver -Ziploc bag -Hotplate -Beakers Temperature (°C) Trial 1 (mL) Trial 2 (mL) Average (mL) 0 120 140 130 10 118 115 116.5 20 118 123 120.5 30 110 115 112.5 40 171 159 165 50 102 92 97 60 0 100 50 70 20 0 10 80 0 0 0 90 0.5 12 6.25 100 0 0 0  Analysis: Looking at the graph above, we notice overall the graph increases to a peak, and then begins to decrease all the way down to zero. At 0 °C the gas production is at approximately 130 mL, however at 10°C, 20°C and 30°C the gas production remains constant- ranging from 116.5mL to 120.5mL and then finally to 112.5mL. At 40°C, there is a sudden boom in oxygen gas production, shooting up to 165mL. After this boom, the amount of oxygen produced decreases at 50°C down to about 97mL. It continues to decrease at 60°C and 70°C going down to 50mL and then 10mL. Finally at 80°C, there is no oxygen gas produced but at 90°C we notice some gas production, 6.25mL. The last temperature at 100°C decreases

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