• Second, you close one end of the tube with a clamp. • Third, you place 10 ml. of solution A,B,C into separate dialysis bags with a pipet. (Solution A- with sucrose, solution B with twice as much sucrose, and solution C containing water.) • Fourth, clamp the other end of tubing, leaving about a 2 cm.
Observe the color change. Osmosis: The materials needed for the osmosis experiment include: 1%, 20%, and 40% sucrose solutions, a 100ml beaker, pipettes, 3 dialysis bags/tubing, scales, and weigh boats. The procedure was a follows; Place the 1% sucrose solution into a beaker. Next, fill one dialysis bag with 1% sucrose
The 15 M NH4OH was added drop wise until a color change occurred, or until 20 drops were added. An additional 10 drops of 15 M NH4OH were then added to each solution. Again, 5 centrifuge tubes were labeled for the same 5 cations and 20 drops of each solution were added to the appropriate centrifuge. HCl was also added to a 50 mL beaker. The Nichrome wire loop was dipped in the HCl solution and placed over the Bunsen burner to disinfect it.
At the bottom of the petri dishes we level it with four quadrants to make sure that each of the antibiotics were placed in the right quadrant and be able to see how the bacteria acted in respect to the placement of the antibiotic. One of the quadrants was kept as the control of the experiment. There were six antibiotic but each group obtain only three of the antibiotic for each bacteria. When placing the bacteria on the petri dish we used a pipette to obtain 100uL of the bacteria onto the petri dish. With a rod (spreader) that was dip in ethanol and then place under the busen burner in order to neutralize it; we spread the bacteria on every surface area of the petri dish.
If they don't line up, pull them out and line up. Do this carefully, as it's the last step before crimping on the connector. Step 8: Carefully insert the connector and cable into the crimping tool, which has two dies that will press into the connector, and push the pins in the connector into the conductors inside the connector. Now the installation is done. After the whole installation, you should check to ensure all conductors are making contact and that all pins have been crimped into their respective conductors.
Put the flask with milk in the beaker which is filled with water, and place it on the wire gauze. Put the thermometer into the milk(under the milk). Turn on the Bunsen burner and warm the milk reach to 50~60 degrees Celsius. 4. The milk was taken out from the water, then 15~20 drops glacial acetic acid were added.
QNT1- Solutions/ Task 2 Given: A chemist needs 500 g of a 5% aqueous HCl solution. Instructions: Using complete sentences, describe how to make this solution. Show how to determine the amount of solute and solvent required, including all mathematical formulas and computations. Results must be labeled and include appropriate units for mass. Assume that a 37% by mass HCl stock solution will be used to prepare the 500 g of a 5% aqueous HCl solution.
Repeat steps 6-8 with the remaining chemicals, including the unknown. 10. All chemicals can be rinsed down the sink. Extinguish the flame by carefully replacing the cap over the flame. Data Table: | Sodium Nitrate | Barium Nitrate | Calcium Nitrate | Cupric Nitrate | Lithium Nitrate | Potassium Nitrate | Strontium Nitrate | Unknown | Flame Color | | | | | | | | | Results and Discussion: 1.
Then, using the pipette fill drop one drop of dish soap into both cups to act as a surfactant or “wetting agent”. Next we have to draw the gases out of the spongy mesophyll tissue and infiltrate the leaves with the bicarbonate solution. To do this, open your syringe and place ten of the leaf disks in one syringe and ten in the other. Once that is complete, take the syringes and fill one with 5cc’s of bicarbonate solution and fill the other syringe with 5cc’s of water (make sure all leaves are suspended and aren’t already floating. Now we have to create a vacuum in the syringe to draw the air out of the leaves.
In the back lab, we mixed the urea water in a big jug and took all the urea water for the conicals out of the jug so we knew that the concentration of urea would be consistent. The dyes we chose were black, bright orange, blue, emerald green, and banana. Next, we measured out how much dye we would need to make the 200%, 100%, 75%, 50%, 25%, and 0% solutions using 50 mL of urea water per each conical. So since we used five different dyes and six different concentrations we required 30 conicals. Once all the dyes were separated by concentration