Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution. Then 5 mL of YY ligand, were poured to each of the five flasks. Each flask had 5 mL of 2M sodium acetate and 4 mL of 3M NH2OH. Then the whole solution was diluted up to the 100 mL fill mark with distilled water. This was the solution that was used in order to obtain the absorption spectrum for each of the different iron (II) ligand examples different flasks.
From the gel electrophoresis, we found that the PCR product was about 600 bps. Spectrometric analysis helped us to find the concentration of amplified DNA which was about 65 μL/mL. The next step was to produce digoxigenin-UTP labelled RNA probe by in vitro transcription of PCR amplified tau DNA with T7 RNA polymerase. About 4 μL of template DNA was added to a sterile, RNase-free reaction vial. The total sample volume was made up to 13 μL by adding water.
The purpose of this experiment was to determine the effects of substrate concentration and pH on the initial rate of enzyme-controlled reaction. METHODS I conducted five experiments. In each experiment, I had five test tubes with constant pH levels of an enzyme solution. The pH levels over the 5 experiments were 3, 5, 7, 9, and 11. I added varying levels of substrate to the test tubes in each experiment.
The preparation was allowed to equilibrate for five minutes. A coverslip was applied and the preparation observed in the microscope. The culture of Paramecium aurelia was mixed. A drop of culture was then placed on a glass slide with an equal volume of Protoslo. A coverslip was applied and the preparation observed in the microscope.
LabAlejandra Lopez 3rd period. LAB PROBLEM How long do onion root tip cells spend in each phase of the cell cycle? OBJECTIVE In this Biolab, you will: * Identify the phases of the cell cycle * Determine the time a cell spends in each part of the cell cycle PROCEDURES * Add the number of cells in each phase and record these numbers in the row marked total. Total the number of cells you counted. * Calculate the number of cells in each phase by dividing the number of cells in that phase by the total number of cells you counted.
2^13-2= 8190 9. Given 3 bits of mask for the host portion, how many host addresses are available? 2^3-2= 6 10. Given 8 bits of mask for the network portion, how many subnets are available? 2^8= 256 11.
2. Record initial pH using the pH paper. Use forceps to dip the small strip into the water and compare the color change to the standard color chart. 3. Add 0.1M HCl one drop at a time.
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).
The volume in cubic feet of a box can be expressed as V(x) = x 3 − 6x 2 + 8x, or as the product of three linear factors with integer coefficients. The width of the box is 2 – x. a. Factor the polynomial to find linear expressions for the height and the width. b. Graph the function. Find the x-intercepts.
c) Name 2 different methods of measuring pH of substances (in a laboratory)? • Probe and meter • Litmus paper 5. Explain the relationship between the natural pH of the skin and the action of: a) Microflora • The bacterial microflora on our skin are able to survive acidic conditions with a pH range 4-6. Our acidic skin protects us from harmful bacteria or pathogens, our microflora are able to breakdown the fatty acid molecules and thereby increase its