Conclusion 1. Explain the relationship between the following words – cells, genes, chromosomes, tissues, DNA, proteins. * * Tissues are made of cells that contain chromosomes made of DNA with regions called genes that code for proteins * 2. Explain why scientists probably used PCR when they prepared the DNA you’re your experiment. * * PCR is the process of copying DNA.
The cells are then treated with a soap solution that degrades the Plasma Membrane and Nuclear Membrane, releasing the cell's contents. The soap also causes proteins and lipids to precipitate out of the solution. Salt is used to stabilize the DNA, which can then be isolated from the solution by adding a layer of ethanol, which causes the DNA to precipitate. Pre-Lab Questions 1. Why is the Banana treated with a soap solution?
Purpose In this lab we are trying to get a broader understanding of the transformation of bacteria by exposing them to pBLU plasmids. Introduction Transformation is the manipulation of a bacterial cell's DNA in order to alter the cell's genotype or phenotype by absorbing free DNA from its surroundings. This can result in a nonpathogenic bacteria becoming pathogenic by absorbing the DNA of a broken open or dead pathogenic bacteria. In our case it is taking in the pBLU plasmid. A plasmid is a spherical self-replicating DNA molecule that is not actually a part of the bacterial cell but can integrate itself into the bacterial chromosome.
c. What would be the sequence of the mRNA after this sequence is transcribed? d. How many amino acids does this portion of Henry's coding stand actually code for? In the first mutation of the CYP2C9 gene described in Henry's story, the 1075th nucleotide has been changed from an adenine (A) to a cytosine (C). This mutation converts an ATT triplet code in the coding strand of the DNA molecule to CTT. Beginning with this triplet code on the DNA, describe the effect that this change would have on the following: a.
Burst the cellular membrane and the nuclei What is the name of the process used to amplify DNA? Polymarse Chain Reaction How is the amplified DNA sorted? By size What # did the DNA profile match? #3 Why was Greg’s DNA profile in CODIS? Because all forensic scientists are required to have their DNA in CODIS Toxicology Lab: Where is vitreous humor normally located?
7. The figure to the right shows that injection of M-phase cytoplasm into an oocyte triggers the cell to enter mitosis. What would be the expected effect on cell division if cytoplasm from a G2-phase cell were injected into an M-phase cell? A. DNA replication would begin again. B. activation of all cyclin-CDK complex in the cell.
All cells run on a set of instructions spelled out in DNA DNA ! Cells ! Bodies ! How does DNA code for cells & bodies? " how are cells and bodies made from the instructions in DNA Regents Biology!
RNA primase lays the beginning for DNA Primase to begin laying down the nucleobases: Adenine, Thymine, Cytosine and Guanine. 3. Okazaki fragment from RNA primase a segment of the lagging strand during replication. 4. DNA ligase goes over all the small Okazaki segments and binds them into a new strand of DNA.
Explain how the process of blanching is used in the food industry and why a specific temperature is required. -Blanching is used in the food industry to quickly heat products containing peroxidase to denature the proteins in an attempt to stop peroxidase from producing the foul-tasting hydrogen peroxide molecules. A specific temperature is required because the food needs to be heated, but not so much so that it gets cooked, or not so little so that the peroxidase remains
Excretory System Parts and their Functions The Liver-The liver detoxifies and breaks down chemicals, poisons and other toxins that enter the body. For example, the liver transforms ammonia (which is poisonous) into urea (which is then filtered by the kidney into urine). The liver also produces bile, and the body uses bile to breakdown fats into usable fats and unusable waste. Bile-After bile is produced in the liver, it is stored in the gall bladder. It is then secreted within the small intestine where it helps to break down ethanol, fats and other acidic wastes including ammonia, into harmless substances.