After that, I placed the slide under the microscope and began searching for the cells that were on the slide. Method: Fish Cells First I chose a slide with fish cells on it, then I placed it under the microscope. I then began searching for the different stages of mitosis in the cells. I then took pictures of the slide, and drew pictures of the the different stages of mitosis. Conclusion In conclusion, after learning to make a cheek cell slide and viewing both the cheek cells and the fish cells in the microscope, I could identify the stages of mitosis.
Microscopes are indispensable instruments because they are used to view such samples and objects that cannot be seen with the unaided eye. Biologists may use them to examine cells, bacteria, and the structures of bones and tissues while Geologists may use them to study rock samples. With Microscopy, Scientists can study atomic structures, cells, tissue and blood cells, which in turn can spot and protect against harmful diseases caused by pathogens. Purpose: The purpose of this laboratory was to improve microscopy skills by practicing the use of microscopes in a lab environment, determining the field of view for low, medium and high objectives in millimeters and microns and examining two tissue samples (blood and a multi-polar neuron). This laboratory allowed individuals to view erythrocytes and leukocytes under different magnification levels.
n. Base: The base supports the microscope. Question: Determine the total magnification given that you are using a compound microscope with the following objectives: 4X, 10X, 40X, and 100X. Assuming that the eyepiece contains a 10X power lens, the objective lens magnification would be 40X, 100X, 400X, and 1000X, respectively. 1. What is meant by the
Microscope and Cells Lab #1 Microscopes and Cells Lab Introduction We used this lab to practice using correct microscope techniques and to note how the samples we observed changed with different magnifications. Purpose The objective of this experiment was to view the “E” slide, amoeba slide, and cheek (multicellular organism) slide through a binocular light microscope at 40x, 100x, and 400x magnification and to identify the specimen. Materials Binocular Light Microscope “E” Microscope Slide Amoeba Microscope Slide Toothpicks Coverslips Methylene Blue Water droppers Methods We used the binocular light microscope to examine the slides at 40x, 100x, and 400x magnifications and recorded what we studied. We viewed the “E” slide first and were able to understand the different magnifications. We then had a teammate use a toothpick to scrape cells from her cheek and swab them on a slide, she placed the metheylene blue on the slide and used the water dropper to rinse the excess off of the slide, after allowing the slide to dry she placed the coverslip on.
We used two methods of determining the focal length of the lenses, estimating the magnification of the convex lenses, and using the lenses to create two different types of telescope. The first way we estimated the focal lengths of the two positive lenses a & b was to form images of a bright object located a long distance away and measured the distance between the lens and the image, which is the focal length. We then recorded our estimated focal lengths in the tables. Another way to estimate the focal length was an equation we used from the lab. Using the values for lens a and lens b, we filled in the columns for image distance in the two tables.
Examination of Simple, Negative, Gram, and Endospore Staining Microbiology – 20122 (A16) Abstract The purpose of this laboratory experiment was to identify a variety of bacterium using various types of staining. We worked with five different types of bacteria and used four different types of staining. Bacteria are prokaryotic cells and are composed of different structures and appendages that can be identified by using specific staining techniques. An important construct of staining is its ability to create a contrast between the bacteria and the surrounding medium. We were able to able to identify the diverse structures through the various staining techniques throughout the laboratory experiment.
Materials In the Materials section of your Lab Report, list the materials (everything) necessary to get this lab done. C. Procedure The Procedure section of your Lab Report lists instructions or directions needed to accomplish the lab. They are already listed on the paper and do not need to be repeated. D. Data In the Data section of your Lab Report, you will be recording “just the facts” you’ve observed in your experiment. Data may include tabulated numbers, graphs, and short explanations of observations.
Hence the concept of holistic approach, which investigate the complex system as a whole and not by splitting it and recombining it as classic reductionist, appear and plays critical role in depicting the near causalities biochemical mechanisms in complex organisms. Together with Darwin theory of evolution, the Mendel theory of unit of heredity did an instant impact in the evolution of molecular biology that eventually set up the platform (indirectly) for its revolution. The discovery of nucleic acids 1944 by Avery et al, the discovery of double helix structure of DNA in 1953 by
DIFFERENT TECHNIQUES USED IN MICROBIOLOGY TO STUDY MICROORGANISMS Different microorganisms have different characteristics therefore their method of study are different accordingly. Following are the few techniques commonly used to study them: 1. LIGHT MICROSCOPY: Microscope is the most used tool in doing research and in identifying microorganism. 1.1 PRINCIPLE: Basic principle used in the light microscopy is that light come from the source which is passed through the specimen through the objective lens whose focal length range from 10x to 100x. This image act as an object for the ocular or eye piece whose magnification power is usually 10x.
The scientific method uses inductive reasoning and deductive reasoning to try to create helpful and dependable models of nature and natural phenomena. In other words scientists use their abilities to obtain information about the world visually or using scientific equipment. This information is written down by the scientist as they observe, her, and feel (McConnell, et al., 2010). We use observations and reasoning in our culture and physical environment. We take these observations and reasoning’s to form ideas, these ideas are known as hypothesis of facts or these ideas are known as hypothesis, “ A hypothesis is a testable explanation of facts or observations” (McConnell, et al., 2010).