Experiment 2: Paper Chromatography
The generic objective of this particular experiment is to learn how chromatography can be used to separate mixtures of chemical substances. Also, this experiment is important because it lectures us that there is an important relationship between the solvent, the chromatography paper, and the mixture. Also, we learned how to work with flammable chemicals and how to dispose them properly.
The procedures conducted during the experiment were; prepare three small beakers where water, acetone and ethyl acetate would be placed (solvents). Then, we took 12 strips of cellulose paper that will be used as the medium. After the strips were gathered, we picked four different colors and with them we put a dot of the pigment (color) three times for every color. At that point, we gathered the colored cellulose strips and submerge them into the solvent without having the solvent touch the colored dot. After this, we observed how the solvent reacted with the pigment, and observe how far the pigment traveled. The pigments that are attracted to the medium more than the solvent will not be able to travel far sticking to the medium to stay near the bottom. In the contrary, if the pigments are attracted to the medium less than to the solvent, they will travel with the solvent towards the top.
Results and Calculations:
Consider Felt-Tip Pens:
Series 1 = Color 1 (Blue), Series 2 = Color 2 (Purple) Color 1 Color 2
What would be the minimum number of pigments each color should contain?
Color 1: minimum numbers of pigments is one
Color 2: minimum numbers of pigments is two
Can you tell the actual number of pigments each pen contains? The pigments were visible after the solvent was able to separate the different pigments. However, if the solvent was not there, then the actual number of pigments