Lab Report

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Amy Stiverson BSC 197: Section 004 Lab 3- Chromatographic Analysis of Plant Pigments Introduction: Photosynthesis is light driven synthesis of organic molecules. It is the reverse reaction of respiration. Plant cells utilize oxygen for glucose breakdown and ATP generation. The overall reaction of photosynthesis is written: 6CO2+6H20----C6H12O6+6O2 Photosynthesis happens in the chloroplasts of a plant cell. In this experiment we will be working with the pigments chlorophyll A, chlorophyll B, beta-carotene, and lutein. These pigments are molecules that absorb light energy and transfer this energy the central chlorophyll. Chlorophyll A and B are the most important pigments that absorb light in the red and blue parts of the spectrum and let off a yellow or green light. Beta-carotene and lutein are accessory pigments because they absorb light in a different region of the spectrum and transfer the energy to the chlorophyll, letting off an orange light. Chlorophyll A and B are very close in molecular structure as is beta-carotene and lutein. In this lab we will be identifying photosynthetic plant pigments from spinach and carrot plants. We will be using paper chromatography to separate chlorophyll A and B and beta-carotene and lutein. Chromatography is used to separate structurally similar compounds in complex mixtures. Chromatography is one of the most commonly used techniques in modern cellular biology. We will be taking the Rf values of the standards tested. Rf=(distance the spot traveled from origin)/(distance solvent migrated from origin) I hypothesize that the chlorophyll A and B will travel the farthest because they are similar in molecular structure and they let off a green light. Methods and Materials: For this lab we will need extracts of spinach and carrot to test. Half the class will also test chlorophyll A and beta carotene and

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