I capped the tube and I mixed until the tablet disintegrates. I wait 5 minutes for the color to develop. Then I compared the blue color of the solution to the potassium color chart: L = 40 lbA/6" soil, M = 80 lb A/6" soil, and H = 160 lb A/6" soil. For the nitrogen I used the pipet to transfer 25 drops of the clear solution above the soil to a square test tube. Then, I filled the tube to the shoulder with distilled water.
Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution. Then 5 mL of YY ligand, were poured to each of the five flasks. Each flask had 5 mL of 2M sodium acetate and 4 mL of 3M NH2OH. Then the whole solution was diluted up to the 100 mL fill mark with distilled water. This was the solution that was used in order to obtain the absorption spectrum for each of the different iron (II) ligand examples different flasks.
After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid. Place another dry test tube in a 50mL beaker and weigh it. Find a bottle of barium iodide and record the name and molar mass. Then, weight out either anhydrous barium iodide or barium iodide dehydrate into this test tube and dissolve is it in 2 mL of deionized water. Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form.
The resulting product was [Co(NH3)5Cl]Cl2 and yield was 4.453g (.017 mol, 84.8%) Distilled water (25mL) was added to concentrated ammonia (5mL) in a 125mL Erlenmeyer flask. The reaction was heated and stirred, then [Co(NH3)5Cl]Cl2 (.0060 mol) was added to the solution. The reaction mixture was vacuum filtered, and the filtrate was cooled in an ice bath. 6M HCl was then added until the solution was neutral to litmus. NaNO2 (.0217 mol) was added to the solution and was allowed to react for five minutes.
Experiment 2 Time | Observations | 5 minutes | Bubbled like sugar | Once salt water was added | Turned soapy white and thick | Equation: METHOD 1) Put 2 cm3 of castor oil into a 250 cm3 beaker and add 10 cm3 of 5mol.dm-3 sodium hydroxide from a measuring cylinder. (Take care when measuring this out and adding it to the beaker to avoid any drips and spills.) 2) Heat the mixture gently over a tripod and gauze, STIRRING CONSTANTLY with a glass rod (otherwise the mixture erupts). Allow to simmer (boil gently) for 5 minutes. Steady the beaker using
Add 0.1M HCl one drop at a time. Use the stirring stick and stir each time a drop has been added. Determine the pH after 5 drops have been added. Repeat the procedure until 30 drops have been added. 4.
Part A: Spectroscopy 1. 0.05g of Iron(III) Nitrate in a 50-ml beaker. Add 17 mL of distilled water to the beaker and mix with a stir rod until the solid is completely dissolved. 2. Obtain another 50-mL beaker and add 0.05g of Chromium(III) Nitrate to the beaker.
5mL of acidified water will be measured, using a graduated cylinder, and will be transferred to the R tube, and will be immediately vigrously mixed with the reactants. Once the solution turns to an orange or red-brown color, a pipet will be used to quickly remove 30 drops of the solution, then transferred to the C tube, and the mixing will resume until the solution is close to room temperature. The solution will be filtered into the P tube, and the solution that is left in the R tube should be washed three times with 1mL of acidified water each time. The water should then be poured into the P tube, leaving the solid in the R tube. Using a test tube holder, heat the R tube over the Bunsen burner, moving the tube in a circular motion until all the water has evaporated.
In this experiment, a chromatography column was pre-created in a plastic tube containing glass beads with microscopic holes and an excess of buffer solution. After draining the initial buffer solution to the top of the column bed, 25 microliters of a hemoglobin (Hb)/vitamin B12 mixture sample were pipetted into the top opening of the column using a P200 pipette instrument. After a few minutes, the mixture began to separate within the column tube. 25 microliters of the buffer solution were then added to the column, using the P200 pipette instrument, to continue the chromatography process and prevent the column from drying. Drops from the column were collected from this point on, using 10 collection tubes labeled 1-10.
CaCO3 + 2HCl -----CaCl2 + CO2 + H2O ( Volume of acid added (L) ) (1.0mol/L) = moles acid added. Moles acid added - moles base added = moles acid that reacted. Moles of acid that reacted / 2 = moles CaCO3 present in sample. Final answer % CaCO3 3. Put exactly 5.0 mL of water in the 10.0 mL graduated cylinder.