Krebs Cycle Essay

398 WordsApr 19, 20152 Pages
The Krebs Cycle occurs inside the mitochondria with the presence of Oxygen, consisting of multiple reactions that produces the final product at the end of the Krebs Cycle. Which is 8NADH, 2 FADH2 and 2 ATP. Our Experiment is going to be based on only one specific reaction in the Krebs Cycle. The Succinic acid to the conversion of Fumarate. Fumaric is catalyzed by succinate dehydrogenase. This occurs when H+ are moving from succinate to the electron carrier and the coenzyme FAD which is reduced to FADH2 Hypothesis: Our hypothesis was that the tube with the highest substrate will have the highest rate of reaction because it would have a higher amount than the substrate and it would be unable to compete. Our null hypothesis was that the tube with the lowest substrate will have the highest reaction. Experimental design: In this experiment we had a different electron carrier instead of the FAD. We use the 2,6 Dichlorophenolindophenol (DPIP) as the electron carrier. When DPIP is oxidized is will be blue while when its in reduced form it will have a clear color. Our mitochondria would be the extra of the lima bean juice that has a live mitochondria. For our experiment we set up four different tubes with the same amount of bean extract except one was boiled. We had the same amount of DPIP except for the blank and different amount of phosphate buffer. We have the same amount of succinate except for tube 4 and no inhibitor except for tubes 3 and 4. After we finished putting all the solution, we then start using the spectrometer. We first caliberated with the blank, then every 2 minutes we keep we add all the samples in the spectrometer until 16 minutes have passed. Are results: we conclude that the Tube 4 had the highest rate of reaction because the absorbance was the lowest . I noticed that even though it had the highest amount of Malonate it still had the

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