Isolation of Eugenol

662 Words3 Pages
ISOLATION OF EUGENOL FROM CLOVES BY DESTILLATION + PUPOSE To perform distillation using a distillation apparatus and isolate eugenol from cloves. + EXPERIMENTAL The apparatus shown in figure was assembled using a 50-mL round bottom flask as the distillation pot. The distillation pot was charged with 2 g of ground cloves and 25 mL of distilled water. The cloves were allowed to soak in the water until thoroughly wetted (about 10 min), then the mixture was distilled, the distillate being collected at the rate of about one drop every 2 – 3 seconds. After about 10 mL of distillate were collected, the distillate was extracted with 5.0 mL of CH2Cl2 (aka DCM), then again with 5,0 mL of DCM. The DCM extracts were combined, dried with small amount of MgSO4, and evaporated to give the product eugenol. Amt. Cloves used 2.0 g Amt. Eugenol isolated 0.15 g 0.15 % Recovery = -------------- x 100 = 7.46% 2.0 IR spectrum: 3518 (OH), 2937.68 (sp2 CH), 2843.18 (sp3 CH), 1637.44 (alkene C=C), 1511.82 (aromatic C=C) cm-1. These values closely correspond with those of an authentic sample. + RESULTS AND DISCUSSION 0.15 g of eugenol was recovered from 2.0 g of cloves. This corresponds to a percent recovery of 7.45%. Although the % recovery seems slightly low relative to the expected 14-20%, the experiment proceeded as planned. There were no spills or other abnormal physical losses. It is possible that the ratio of the size of the glassware to the theoretical amount of eugenol which can be obtained from cloves in this experiment is large, leading to adherence of a large percentage of the product on the sides of the glass apparatus. If this is so, then steam distillation of a larger sample of cloves should give an improved recovery. Otherwise, it can be concluded that the specific sample of cloves used contains

More about Isolation of Eugenol

Open Document