Isolation, Identification and Characterization of Escherichia Coli from Urine Samples and Their Antibiotic Sensitivity Pattern Essay

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Available online at www.pelagiaresearchlibrary.com Pelagia Research Library European Journal of Experimental Biology, 2011, 1 (2):118-124 ISSN: 2248 –9215 Isolation, Identification and Characterization of Escherichia Coli from Urine Samples and their Antibiotic Sensitivity Pattern Manaal Zahera*, Chetan Rastogi, Pushpendra Singh, Sana Iram, Shumaila Khalid and Akhilesh Kushwaha Institute of Transgene Life Sciences, C-4/1, Vikalp Khand, Gomtinagar, Chinhut, Lucknow, U.P ______________________________________________________________________________ ABSTRACT A urinary tract infection is an infection that begins in your urinary system. Urinary system is composed of the kidneys, ureters, bladder and urethra. Though Escherichia Coli, which is normally present in the gastrointestinal tract is the commonest causative organism, other gram negative colonic bacteria. Any part of your urinary system can become infected, but most infections involve the lower urinary tract, the bladder and the urethra. Women are at greater risk of developing a urinary tract infection than are men. A urinary tract infection limited to your bladder can be painful and annoying. However, serious consequences can occur if a urinary tract infection spreads to your kidneys. Antibiotics are the typical treatment for a urinary tract infection like (Norfloxacin, Tetracycline, and Ampicillin etc) .By Antibiotic sensitivity pattern we can measure the Zone of diameter in millimeters. The size of zone of inhibition was interrupted by referring to the CLSI (Clinical Laboratory Standard Institute) guidelines and organism was labeled susceptible, intermediate, or resistance accordingly. Key words: Urinary Tract Infection, Gram negative Rod shaped, Escherichia species Antibiotic sensitivity pattern, Norfloxacin, Tetracycline, Ampicillin, Zone diameters in millimeters.

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