The total sample volume was made up to 13 μL by adding water. The reaction vial was placed on ice, and was added 2 μL each of 10x NTP labeling mixture, 10x transcription buffer, and T7 RNA polymerase. 1μL of protector RNase inhibitor was also added, and the contents in the vial were mixed gently and incubated for 2 hours at 37 degree Celsius. To remove the template DNA after transcription, 2 μL of DNase I was added and incubated for about 15 minutes at 37 degree Celsius. The reaction was stopped by adding 2 μL of 0.2 M EDTA at pH 8.
Add 1 mL of deionized water to the small test tube containing the precipitate and mix it and centrifuge it for 60 seconds. Then, add the supernatant into the boiling test tube and repeat this step one more time with another 1 mL of deionized water. Acquire a pair of metal test tube holders and heat the boiling test tube to evaporate the water for 15 minutes. Let is cool after and weigh it. Then, calculate a percent yield of zinc iodide and write a balanced chemical equation and determine the limiting
During week 2, the process we performed, was called distillation. Distillation is when we set up a Distillation Apparatus and used a Bunsen Burner to complete the process. The alcohol part of the solution boils at 78oc. The water part of the solution boils at 100oc. Both solutions were under normal vapor pressure.
Juliana Park Mayumi Tamada CHEM 111B LAB/ M-F 1-4PM 15 August 2012 Spectroscopy Lab Introduction In this lab, the molar absorptivity of the complex FeLn2+ will be determined by using the absorbance of the complex and its concentration. The absorbance will be found by using a spectrophotometer. For the next part of the lab, the formula of the complex will be determined by also using the volume of ligand and the absorbance again. Experimental There are two different parts to the experiement. In the first part, five 100 mL flasks of 5 mL ligand solution, 5 mL 2 M sodium acetate, 4 mL 3 M NH2OH, and 1-5 mL Fe2+ solution are diluted with water.
Repeat steps 1-5 for trial 2. Variables and Control test: Independent Variable: Amount of each reactant poured into the test tubes Dependent Variable: Height of each solution Constant variables: Amount of time waiting for solution Data Table: Test tube # | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Trial 1 | 0.5 cm | 1.0 cm | 1.1 cm | 1.5 cm | 1.5 cm | 1.5 cm | 1.3 cm | 1.5 cm | Trial 2 | 0.5 cm | 0.7 cm | 1.0 cm | 1.0 cm | 1.3 cm | 1.7 cm | 1.9 cm | 1.5 cm | Observation/Analysis: Solution turns yellow when a separate product is added, solid of the solution leaks down to the bottom after 20 minutes. Conclusion: The group tried to find the excess or limiting for the reaction between KI and Pb. In the end the hypothesis was supported by the data. We found that little amounts of each product led to a greater height of solids.
For every 20 drops of solution you will add 0.1g of zinc to the new test tube. Repeat steps 3 and four until the solution is clear. If there ever exists too little of the solution to get enough drops, add up to 1mL of distilled water to the solution. 4. Once the solution is clear, retrieve at least ten drops of the solution and place them in a new test tube.
We let them soak for 20 minutes than we removed them from the test tubes. Next we measured their new weights and recorded them. They were; 10% NaCl decreased by 4%, 15% NaCl decreased by 8%, and 20% NaCl decreased by 12.5%.The results showed us the conclusion that the potatoes were hypertonic to the deionized water and were hypotonic to the sodium chloride solutions. Introduction: The objective of this experiment is to detect diffusion and osmosis in potato cells in 3 solutions. Diffusion is the spontaneous spread of molecules from an area of high concentration to an area of low concentration.
The experimenter used 3 identical plastic cups and filled one with room temperature tap water, one with warm tap water and one with boiling tap water. Each cup was placed into the freezer and was checked every 15 minutes for signs of freezing. The experimenter found that the room temperature water showed the first signs of freezing and in the final outcome froze solid before the water in the other cups. His findings showed that water containing impurities, such as tap water, will freeze faster based on a lower starting temperature. The second case study was covered in an article by Science News.
Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution). The supernatant was then centrifuged at 9000 x g and 40C for 15 min and 5 ml of the second supernatant was transferred to a conical tube. The obtained second pellet was resuspended in 4 ml of distilled water and transferred into another dialysis
Tremulousness & Hallucinations This self-limited condition occurs within 2 days after cessation of drinking and is characterized by tremulousness, agitation, anorexia, nausea, insomnia, tachycardia, and hypertension. Confusion, if present, is mild. Illusions and hallucinations, usually visual, occur in about 25% of patients. Treatment with diazepam, 5–20 mg, or chlordiazepoxide, 25–50 mg, orally every 4 hours, will terminate the syndrome and prevent more serious consequences of withdrawal. 2.