Glucose Lab Report

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LABORATORY REPORT Activity: Name: Instructor: Date: Blood Glucose Regulation Miranda Parker Jill Schmitt 10.03.2014 Predictions Plasma glucose levels will be highest immediately after the meal (0 hr) Plasma ketone levels will be highest before the meal (fasting) Plasma insulin levels will be highest 1-3 hours after the meal Plasma glucagon levels will be highest 1-3 hours after the meal Materials and Methods Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon Independent Variable food and beverage intake Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI 4. Why were physical activity and caffeine and alcohol intake the controlled variables? Because you can control on the amount of time you spend doing physical activities and the intake of both caffeine and alcohol. 5. What type of assay was used to measure plasma glucose and ketone levels? glucose, oxidase, peroxide, dianisidine 6. What type of assay was used to measure plasma insulin and glucagon levels? glucose, oxidase, peroxide, dianisidine Results Table 2: Glucose and Ketone Concentration in Blood Plasma Fasting Immediately post meal I hr post meal 2 hr post meal 3 hr post meal Sub. 1 5.2 6.4 6.2 5.7 4.6 Glucose (mmol/l) Sub. 2 Sub. 3 5.3 5.2 6.4 6.5 6.4 5.6 4.6 6.4 5.7 4.7 average 5.2 6.4 6.3 5.7 4.6 Sub. 1 120 107 60 77 46 Ketones (µmol/l) Sub. 2 Sub. 3 112 124 96 98 57 57 68 62 62 54 average 119 100 60 65 56 Laboratory Report/ Miranda Parker/ Blood Glucose Regulation/ Jill Schmitt/ 10.03.2014/ Page [1] of [4] Glucose Concentration in Blood Plasma Ketone Concentration in Blood Plasma 1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest. Immediately post meal and one hour post

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