Glucose Lab Essay

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LABORATORY REPORT Activity: Name: Instructor: Date: Blood Glucose Regulation Jamie Congrove Nicole Chatman 01.25.2015 Predictions Plasma glucose levels will be highest immediately after the meal (0 hr) Plasma ketone levels will be highest before the meal (fasting) Plasma insulin levels will be highest 1-3 hours after the meal Plasma glucagon levels will be highest immediately after the meal (0 hr) Materials and Methods Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon Independent Variable food and beverage intake Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI 4. Why were physical activity and caffeine and alcohol intake the controlled variables? Because of the way the can effect your blood suger 5. What type of assay was used to measure plasma glucose and ketone levels? enzymatic analysis 6. What type of assay was used to measure plasma insulin and glucagon levels? radiommunassay Results Table 2: Glucose and Ketone Concentration in Blood Plasma 5.3 6.5 6.3 5.6 73 Sub. 1 5.3 6.5 6.3 58 70 Glucose (mmol/l) Sub. 2 Sub. 3 5.3 5.3 6.5 101 75 57 62 59 57 67 average 107 101 47 60 4.7 Sub. 1 122 98 60 4.7 73 Ketones (µmol/l) Sub. 2 Sub. 3 111 113 100 6.3 5.6 5.6 4.7 4.7 70 57 average 6.5 6.4 5.7 4.7 67 Laboratory Report/ Jamie Congrove/ Blood Glucose Regulation/ Nicole Chatman/ 01.25.2015/ Page [1] of [4] Glucose Concentration in Blood Plasma Ketone Concentration in Blood Plasma 1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest. post meal and 1 hour post meal at 6.5 pmol/l post meal was the highest 2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma

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