Glucose travels in the plasma or liquid part of your blood but cholesterol (triglycerides) travel bound to protein carriers in the blood. Based on their respective chemical properties, explain the reason for this difference. (2 marks) Cholesterol is an amphipathic molecule, since it has polar and non – polar regions. Even though the cholesterol has polar group (OH) it dissolves fats than in aqueous
Blanca Mbuto 02/19/2012 Biochemistry Lab 4422 Cell Fractionation by Centrifugation Abstract This experiment was performed to investigate organelles, membranes, cellular components, and enzymes. Our concentration was to observe and determine if the chlorophyll contained broken chloroplasts or intact chloroplasts extracted from spinach leaves. A percoll density gradient was prepared followed by the separation of organelles. Chlorophyll was analyzed by using a spectrophotometer. We compared the absorbance readings of each tube and selected three (3) tubes to be analyzed.
Enzymes Amylase: | Tracing the Breakdown of Starch | Rashaud Pickering, Ashley Bagnis, Stephanie Alvarado, Elbany Angulo 3614098 Section U-14 | Signature___________________ Abstract An experiment was conducted to find out what the optimal temperature for amylase to break down starch was. An Iodine test was used to find evidence of starch formation. Three drops of iodine were dropped into two sets of spot plates. There were four test tubes that contained human amylase and four test tubes that contained fungal amylase. These specimens were all tested before being placed into their respective ice or water baths.
Gel Filtration of Proteins Session 4 Lab Report Semester 2 Victoria Franks 2930499 Gel Filtration of Proteins Introduction The aim of this experiment was to separate a range of proteins from a single sample, using their differing sizes to tell which protein was which when passed through a gel barrier. This method is called size exclusion chromatography. A range of coloured proteins and dyes are used so that samples can be easily idenitifed by the naked eye; however, two of the proteins are a similar colour, making it necessary to use spectrophotometry to tell the difference between them, based on the fact that they have different levels of absorbancy. A gel called Sephadex is used within a tight column and the protein sample is passed through using a strong buffer to help the smaller molecules of the protein sample to diffuse into the gel. As the Sephadex forms a network with tiny holes, this prevents molecules that are too large from getting inside.
One of the important aspects of organic chemistry is being able to take an impure mixture of organic molecules, identify it as a mixture and purify each component. In this lab, we will explore several methods of the identification and purification of organic compounds. As these methods will be used throughout the semester, it is important that you learn how to effectively use these methods. Purpose of Experiment 1. Identify that your Excedrin is a mixture of organic molecules using thin layer chromatography (TLC), melting point (mp) and Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR).
Proteins are extracted from animals and humans as a mixture in a serum of body fluids. When immunologists want to study a specific protein, like an antibody, hormone, or enzyme, they need to separate it from the mixture. The technique is used for the separation of sugar, Polypeptides, Proteins, Polystyrenes, Silicones, Liquids, Butyl rubbers and polyethylenes. Types: MEC is a one of several types of HPLC. There are basically two types of MEC.
Digestive enzymes are hydrolytic enzymes. Their substances, or the molecules on which they act are organic food molecules which they breakdown by adding water to the molecular bonds, thus cleaving the bonds between the subunits or monomers. Digestive enzymes can function outside the body cells; their activity can be studied by test tubes (Marieb and Mitchell 2010). This experiment attempts to re-create the breakdown process that is normally done via digestion with Iodine as a vital component. It can be expected that once amylase reacts with the starch, maltose will then be broken down and less starch will be visible and more sugar will be apparent thus causing the solution mixed with iodine to become lighter and lighter.
The compounds in the sample will react with phenol to produce a yellow-gold color, which can then be measured using a spectrophotometer. The results are expressed in terms of a single carbohydrate, usually glucose. Single carbohydrates are digestible and are absorbed in the body to provide metabolic energy. Materials and Methods This experiment was conducted as described in the laboratory manual (Roberts 2011) with the following minor modifications. Firstly, the determinations for the standard curve are done in duplicate in large test tubes.
With the submersion of the cubes in acid the differentiation will be key in proving that each of the cubes has a different absorption rate. With the differences in size they will all be based on different ratios so the rates of absorption for the acid will be different per cube. Part 2 The creation of cells with pre soaked dialysis tubing is a key factor in mimicking a real cell’s interior resources. “Artificial cells made of dialysis tubing or baggies make excellent macroscopic representations for both diffusion and osmosis.” (Friedrichsen)Once the dialysis cells are filled the exterior environment is then introduced with a waiting period of half an hour used to allow for the exchange through the membrane. After the conclusion of the waiting period the cells were then analyzed and to eradicate any inconsistencies were
This will keep both concentrations equal and will also keep the mass the same. 10% Salt-Water Solution: Since the concentration of solutions is higher on the outside is higher than inside the potato cell, the cell will put water on in the solution outside. The potato