Free Radical Case Study

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Free radicals are the main cause of various degenerative diseases like cardiovascular diseases, Parkinson’s disease Alzheimer’s disease (Davis and K.j,2000).To avoid the harmful effects of free radicals there is a need of natural antioxidant system which are obtained from fruits, vegetables, spices etc they showed ability to neutralized these reactive species ((Pihlanto et al., 2008).Medicinal plants were used for centuries as medical remedies as in the form of food, folk medicine or tea (Summer,2000).Herbal tea is made up of leaves, roots ,flowers of various plant species as compared to normal tea which is obtained from single plant Camellia sinensis (Deetae et al.,2012).Consumption of herbal tea is famous worldwide as it is…show more content…
Rosa canina commonly called as rose hip which become popular for researchers as it property to showed remedy in traditional folk medicine due to high content of phenols (Chrubasik et al.,2008).In this study R canina showed strong free radical scavenging activity against DPPH which were able to reduce the purple color into pale yellow color .The other two tea extracts were also showed ablity to discoloration of DPPH .These exracts showed good potent to donate an electron with IC50 values R canina (54.30µg/ml),M alba (79.40µg/ ml) and (110.06µg/ml) for T pratense. However the activity of these extracts was not strong as butylated hydroxytoulene which were used as…show more content…
ABTS is mixed with potassium persulphate to generate the free radical and Nitrites were produced when sodium nitoprusside were mixed with distilled water. The three herbal tea extracts were also able to reduce the blue color of ABTS solution into neutral color with their scavenging activity (51.63 to 81.62%) for R canina , (28.42 to 66.91) T pratense and( 43.85 to77.82%) of M alba. R canina in nitric oxide assay also showed strong radical scavenging activity to reduce the production of nitrates as compared to other two extracts ranging from R canina (48.09 to 80.03 %) T pratense (35.45 to 68.70%) and (38.25 to 72.39±%) for M alba .Lipid peroxidation is an mechanism to damage cellular injury and used as indicator of oxidative stress(Esterbaur et al., 1991).Poly unsaturated fatty acids are damaged by free radicals and create a process of chain reaction which is divided into three phases namely initiation propagation and finally termination (Suresh and Annam,2013)..In general the examined extract of R canina expressed strong antioxidant activity to stop damage of lipids. The largest inhibitory activity again was exhibited by Aqueous extract of R canina with IC50 values(62.65µg/ml) followed by M alba (92.10µg/ml) and least inhibitory activity was showed by T pratense (119.51µg/ml).BHT was used as positive control in all the in
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