Fd & C Dyes Lab Report

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4. By law, company Q must have an M concentration of 3.00 x 10-4 mol/L +/- 5%. In other words, the M concentration must be between 2.85 x 10-4 mol/L and 3.15 x 10-4 mol/L. Does Batch 021015 meet legal requirements? Part II: Colorimetry of FD & C Blue # 1 Dye (Brilliant Blue FCF) Discussion and Review: In this part of the experiment, we will use a color standard calibration system to determine the concentration of an unknown solution. FD&C dyes refer to specific commercial dyes approved for human consumption under the US government’s Food, Drugs, and Cosmetics Act. We will start with FD&C Blue #1, a commercial food dye of known concentration. Through serial dilutions we will end up with a color “standard”, a set of diluted…show more content…
Hands-On Labs, Inc. LabPaq: CK-S Page 117 The National Academy of Science reports that the Average Daily Intake (ADI) of Blue #1 dye is 16 mg. Its chemical formula is: C37H34N2Na2O9S3, and its molecular weight is: 792.84. The formal chemical name is: Disodium alpha -(4-(N-ethyl-3-sulfonatobenzylamino) phenyl)-alpha-(4-N-ethyl-3- sulfonatobenzylamino, cyclohexa-2,5-dienylidene) toluene-2-sulfonate Typically, the concentration of the FD&C Blue #1 is approximately 0.026 M. You will need to dilute it to make a series of standard solutions of known concentrations. Your starting dilution should be 1:1000 (i.e., 1 part dye and 999 parts water). PROCEDURE: Completely read all instructions and assemble all equipment and supplies before beginning work on this experiment. 1. Preparation of the standard: a. Fill a beaker to the 100-mL mark with distilled water. b. Add 2 drops (approximately 0.1 mL) of Blue #1 dye and stir. This will give you a starting concentration of approximately 2.6x10-5 M. c. Calculate and record the concentration of this “starting” solution in

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