It selectively filter ions and organic molecules; controls the movements of substances in and out if the cell. 3) In what type(s) of organisms would the Gram stain not work? Why? - Because Gram staining process target specific on cells that has peptidoglycan, cell membrane, and cell wall so organisms that doesn’t have such will not be detected by Gram staining process, organisms such as virus. 4) How is the Gram stain reaction by bacteria useful information to medical doctors or microbiologists?
I. Title: pGLO Transformation Lab II. Purpose; We want to be able to observe, comprehend and analyze genetic transformation as we genetically alter organisms. III. Hypothesis: If bacteria with + pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies with survive and grow on the transformation plates that have LB/ amp.
Competitive meaning it would bind to the active site therefore blocking it decreasing enzyme activity, noncompetitive being that it would just simply bind elsewhere and or change the shape of the enzyme causing no feedback. For Example if you take too methanol then you will be given ethanol which is a competitive inhibitor of methanol. This way they would both be competing for the active site, thus blocking the active site from the methanol. As for this lab PTU (phenylthiourea) was tested and will find out at the end of the experiment if it would be a competitive or not competitive inhibitor to Catechol Oxidase (Potato Extract). Will the PTU affect the Catechol Oxidase (Potato Extract) and change colors if the liquids are together.
What did those mangy tagi do? What are you going to do to relieve this headache? How will you survive……. Purification of Organic Compounds Thank goodness that you are in organic chemistry lab and not in the South China Sea. One of the important aspects of organic chemistry is being able to take an impure mixture of organic molecules, identify it as a mixture and purify each component.
Pseudomonas Aeruginosa The purpose of this lab was to identify the unknown bacteria culture given by our respected instructor using various differential tests. The identification of this unknown culture was accomplished by separating and differentiating between possible bacteria based on specific biochemical characteristics. Each test was used in a way to help recognize those specifics and bring us one step closer to identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, lactose and glucose fermentation, citrate, indole, urease, motility, H2S, EMB and MAC. The tests performed on the unknown bacteria culture were all used to determine and identify the bacteria.
h) A way to make hard water softer is to put an sodium nitrate and create a precipitate to mellow out the reaction. Another way of making it softer is by removing the calcium ions one way of doing that is by boiling the solution to take out some of the ions. Conclusion: Overall, we determined that sodium carbonate, Na2CO3, is the anion that can be used to precipitate the most metal cations. Also, we learned that the anion sodium chloride, NaCl, could be used to remove silver ions from solutions. The stuff that I found interesting was that how many colours you can get when you mix the cations and anions
Lab Report: Enzyme Lab Your Name: Samuel Thohammer__________________________________________________________________ Purpose of this Lab What is the goal of this lab? What question is it trying to answer, or what problem is it trying to explain? It is going to explain how a liver's enzymes break down substances that could be harmful.| Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. Give your reasons and outline any assumptions that lead you to this hypothesis. I'm guessing that the liver will turn the peroxide into something else, or make it change.| Experimental Design List the materials used in this lab, and the procedure you followed.
Digestive enzymes are hydrolytic enzymes. Their substances, or the molecules on which they act are organic food molecules which they breakdown by adding water to the molecular bonds, thus cleaving the bonds between the subunits or monomers. Digestive enzymes can function outside the body cells; their activity can be studied by test tubes (Marieb and Mitchell 2010). This experiment attempts to re-create the breakdown process that is normally done via digestion with Iodine as a vital component. It can be expected that once amylase reacts with the starch, maltose will then be broken down and less starch will be visible and more sugar will be apparent thus causing the solution mixed with iodine to become lighter and lighter.
Enzymes Amylase: | Tracing the Breakdown of Starch | Rashaud Pickering, Ashley Bagnis, Stephanie Alvarado, Elbany Angulo 3614098 Section U-14 | Signature___________________ Abstract An experiment was conducted to find out what the optimal temperature for amylase to break down starch was. An Iodine test was used to find evidence of starch formation. Three drops of iodine were dropped into two sets of spot plates. There were four test tubes that contained human amylase and four test tubes that contained fungal amylase. These specimens were all tested before being placed into their respective ice or water baths.
With the submersion of the cubes in acid the differentiation will be key in proving that each of the cubes has a different absorption rate. With the differences in size they will all be based on different ratios so the rates of absorption for the acid will be different per cube. Part 2 The creation of cells with pre soaked dialysis tubing is a key factor in mimicking a real cell’s interior resources. “Artificial cells made of dialysis tubing or baggies make excellent macroscopic representations for both diffusion and osmosis.” (Friedrichsen)Once the dialysis cells are filled the exterior environment is then introduced with a waiting period of half an hour used to allow for the exchange through the membrane. After the conclusion of the waiting period the cells were then analyzed and to eradicate any inconsistencies were