Experiment on Lipids

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Experiment 3: Lipids Members present: Dawnie April Rose A. Jamaca, Leslei B. Potutan Locker No. 85 Chemistry Department, Xavier University-Ateneo de Cagayan, Cagayan de Oro City Date Performed: August 15, 2014 Date Submitted: August 18, 2014 ABSTRACT The experiment “Lipids” is to differentiate glycerol, cocnut oil, lecithin, and oleic acid in terms of their chemical properties as lipid substances. Several tests were done in order to properly distinguish each lipid from one another. Acrolein Test, Test for Unsaturation, Test for Phosphate, Emulsification Test, Lieberman-Burchard Test, Carr-Price Reaction and Modified Furter-Meyer Test were performed in order to achieve the objective of the experiment. The four of the samples are all lipids but have different characterisitcs INTRODUCTION Fats and oils are included in the class of Lipids. They have a fairly simple structure starting with the 3-carbon glycerol molecule. Each carbon picks up a bit called a fatty acid. Phospholipids, such as lecithin, have a polar portion and a non polar portion consisting of the long chain fatty acids within the same molecule. Consequently, the polar portion of these molecules will mix with water and the non polar portion repels the water but mixes with lipids. The phospholipids will form small globules in water that make the water look opaque or milky. When lipids mix with water, this is known as emulsification and the mixture is known as emulsion. Cholesterol reacts with acetic anhydride when sulfuric acid is used as a catalyst for dehydration, to form a series of colors from red through violet and blue-green.(1) Lipids are also used for insulation in organisms that need to hold onto heat in unusual conditions. The fatty acid residues may differ in chain length and be distinguished by the difference in intensity of color due to the absorption of Sudan III. The fatty

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