Allow the mixture to cool for a few minutes then filter it, using either gravity or vacuum filtration. (We shall be using vacuum filtration.) Wash the residue in the funnel once with a little water and collect all the filtrate. 4. Pour all the filtrate and washings into a 250cm3 volumetric flask.
Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated. During this procedure, the iodine extract was filtered and several tests, both standard procedure and home- Objective: The purpose of this experiment is to understand how a single substance can be isolated from a mixture, like seaweed and water. Also, learn how to utilize filtration and extraction to make a tincture for testing for Iodine, Iodide Ion, and Triiodide Ion. Procedure: 1. 6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water.
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
Vancomycin-Resistant Enterococcus - Enterococci bacteria are normally found in the intestinal tract. They can sometimes become pathogenic and develop resistance to vancomycin. Vancomycin is considered to be the last-resort antibiotic that is administered for infections that are resistant to penicillin and other antibiotics. Vancomycin-resistance decreased because the antibiotic was no longer used and the resistant strains were no longer selected for. With recent outbreaks of MRSA (Methicillin-resistant Staphylococcus aureus) VRE demonstrates that decreasing use of antibiotics can decrease the drug resistance in bacterial
It is expensive and has serious side effects. It must be administered intravenously because it is ineffective if given PO (http://goapic.org/MRSA.htm). Vancomycin is metabolized by the liver and excreted by the kidneys. Each individual metabolizes differently and this may be further compounded by disease, illness, age and sex, so trough levels need to be monitored to prevent toxicity and/or damage to major organs (Williams & Hopper, 2007 pg 105). There is fear that further mutation will cause resistance to all currently available antibiotics, including Vancomycin.
Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form. Then, centrifuge it for 1 minute. On the side, weight a boiling test tube containing 2 boiling chips. When the separation is complete, remove the small test tubes from the centrifuge and decant the supernatant into the boiling test tube. Add 1 mL of deionized water to the small test tube containing the precipitate and mix it and centrifuge it for 60 seconds.
Measure approximately 1 g of Copper(II) Sulfate Hydrate into the crucible and crucible and lid. 4. Heat the crucible gently. 5. Stirring carefully and observe the color of the hydrate until it changes to a consistent white color, then the Copper(II) Sulfate is dehydrated.
The contents were disposed of and rinsed with distilled water. For the flame confirmation tests, 20 drops of each metal solution were added to their appropriate tubes. A Bunsen burner was lit and adjusted till a blue cone appeared in the flame. A platinum loop dipped in HCl was placed above the
Having researched into two antiseptics, I have decided that TCP and Dettol antiseptics were likely to give me some sort of effective data in stopping bacterial growth in which I will have data through my experiment. After having completed my experiment I will be able to identify which antiseptic is the most effective in stopping bacterial growth, providing my client group with the best antiseptic to use within their work place in fighting infections and help within the first aid assistance in the emergency department within the
White precipitate shows the presence of chloride (Cl-). Chloride anion equation: HCl(aq) + AgNO3 (aq) → HNO3 (aq) + AgCl(s). The nitrate anion test involves cooling a mixture containing 1 mL of test solution and 3mL 18M H2SO4. 2mL is poured down the inner test tube side and the presence of a brown ring shows nitrate (NO3-) to be present. The carbonate anion test mixes 1 mL of test solution and drops of 6M HCl.