DETERMINING THE PROPERTIES OF AN ENZYME I. Abstract Enzymes are responsible for the speed at which chemical reactions they are involved in take place. This experiment determines the effects that concentration, temperature, pH, and boiling have on an enzyme’s ability to perform its work. It is hypothesized that none of these variables will have any effect on the activity of enzymes and these hypotheses are tested using dye-coupled reactions to determine the rate at which peroxidase converts H2O2 into water (H2O) and oxygen (O2). Each hypothesis is subsequently rejected as data suggests that concentration, temperature, pH, and boiling all have an effect on enzyme activity. II.
ENZYME worksheet 1. Label the diagram [pic] a. substrate b. active site c. enzyme d. products 2. Answer true of false to the following statements: ___T____ Enzymes interact with specific substrates ___F____ Enzymes change shape after a reaction occurs ___T____ Enzymes speed up reactions. ___F____ One enzyme can be used for many different types of chemical reactions. ___T____ Enzyme reactions can be slowed or halted using inhibitors.
Make sure to keep time, read the spectrometer, and record the data. Note time to the nearest second and mix the contents of tubes 2 and 3 by pouring them back and forth twice. Mixing should be completed within ten seconds. 5) Add the reaction mixture to a cuvette by pouring or using eye dropper, wipe the outside, and place the cuvette in the spectrometer. Read the absorbance at 20 second intervals from the start of the mixing.
The absorption spectrum is measured using a spectrophotometer and the data is graphed in Excel. The peak of the line is used to find Vmax of Fe2+. Vmax is used to find the moles of Fe2+ and ligand. The unknown n is a ratio of moles ligand divided by moles Fe2+. Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution.
Lab # 4 : The Redox Arena II Procedural Summary There will be three different sized test tubes that will be used in this experiment, a boiling tube, a large and small test tube. The boiling tube will be labeled “R” for “reactants”. A scale with a weighing paper will be tared before adding 2 grams of granular zinc (Zn), record the mass, and then move the zinc to the boiling (R) tube. Weigh out 2 grams of iodine crystals, record it, and this will be added to the R tube, with the zinc. The R tube will then be put in a large beaker.
Lactase Buffer solutions 4,7,9 Enzymes are organic catalysts that control the rate of chemical reactions in cells while not being permanently altered themselves. In general, enzymes speed up the rate of reaction by lowering the activation energy required to start reactions. Lactase is an enzyme used by the body to hydrolyze lactose, a disaccharide unique to mammalian milk, into the monosaccharaides, glucose and galactose. Lactose has been shown to aid in the absorption of several minerals, including calcium, magnesium, and zinc. .
(2 marks) A drug of same size and shape, as that of a substrate, which fits into the active site of an enzyme will act as a competitive inhibitor and the reaction is termed as competitive inhibition. The drug will compete with the substrate molecule for the active site of an enzyme, thus, it will decrease the rate of substrate-enzyme reaction by binding to the active site of enzyme. The rate of competitive inhibition reaction is directly proportional to the concentration of inhibitor present. 2. Glucose travels in the plasma or liquid part of your blood but cholesterol (triglycerides) travel bound to protein carriers in the blood.
Hereditary Fructose Intolerance and Mitochondrial Disease Sara James Western Governor's University Hereditary Fructose Intolerance Enzymes in Breakdown of Fructose Enzymes are proteins that carry out chemical reactions. They will bind to a substrate and then end up releasing a product. The enzymes do it by a process of lock and key. The lock is considered the substrate and the enzyme is considered the key. Only the enzyme will fix the substrate because of the active sites on the enzyme.
Explain what it means to denature an enzyme. -To denature an enzyme means to change its shape by breaking the hydrogen bonds that holds its shape, thus rendering it useless. An enzyme can be denatured with heat, or with a change in pH. 5. What factors did you need to control in your tests?
They are used to speed up the reaction rates by providing an alternative reaction pathway of lower activation energy, which is the minimum energy that requires for reactions occur. Catalysts take part in a reaction (chemical involved), but they do not undergo any changes in chemical reaction. (Farabee, 2001) Enzymes are proteins made of a combination of different amino acids. Therefore, the enzymes may be lost