Enzyme Lab Essay

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All living organisms have enzymes. Enzymes are globular proteins that selectively speed up (catalyze) the rates of chemical reactions. In our experiment we used yeast which contains catalase, our enzyme, which will react with our substrate, hydrogen peroxide thus making oxygen gas that we can measure. In our first experiment we examined hot and cold temperatures impact on catalase activity our research question was, ‘what is the relationship between temperature and yeast catalase activity?’ Our hypothesis was: If we test cold and hot temperatures, then colder temperatures will have more catalase activity than hotter temperatures. In our 2nd, we examined the effect of warmer temperature. Our research question was, ‘does catalase denature in stages or all at once as temperature increases?’ Our hypothesis was: if temperature increases, then the catalase will denature in stages as the temperature increase, the catalase will slowly stop working. We followed the procedures in the lab manual (choinski and karatit 2014) with the following expetions: For experiment 1, we used 1.5% hydrogen peroxide concentration and experimented this concentration at 4*c, 24*c (room temp.), 44*c, 52*c and 60*c. For experiment 2, we used 3% hydrogen peroxide concentration and experiment this concentration at 21*c (room temp.), 35*c, 45*c, 55*c, & 60*c. Figure 1: Effect of temperature on catalase activity. From 4*c to 52*c there was a -44.1% decrease in catalase activity but had a 1.6% increase from 52*c to 60*c. (N=3 treatment) Figure 2: Effect of temperature on catalase activity from 21*c to 35*c there was a -151.4% decrease in catalase activity and rom 45*c to 55*c there was a -55.9% decrease. (N=temperature) Experiment 1 investigated the effects on cold and hot temperatures on catalase activity. Between 4*c to 52*c there was a decrease of -44.1% in the catalase activity. The

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