Then both test tubes were placed in a room temperature bath. Then 3-4 drops of iodine were added and adjusted. After 5 minutes, the position of the dye was recorded and then after every 2 minutes for 20 minutes the dye position was recorded. Time (minutes) Dye Position Test Tube #1 pt 30OC Test Tube #2 pt 100OC 0 0.1 0.1 2 0.1 0.1 4 0.1 0.1 6 0.11 0.1 8 0.11 0.1 10 0.12 0.1 12 0.12 0.1 14 0.13 0.1 16 0.14 0.1 18 0.14 0.1 20 0.15 0.11 Figure 1: Each test tube contained 20 pea seeds which were pretreated for 15 minutes in 0.2M sucrose solution at 30OC and 100OC. Each test tube contained a cotton ball dampened with 15% KOH, then a ball of glass wool, then 20 pea seeds soaked at the certain temperature, then another ball of glass wool, and then another cotton ball dampened with 15% KOH.
Juliana Park Mayumi Tamada CHEM 111B LAB/ M-F 1-4PM 15 August 2012 Spectroscopy Lab Introduction In this lab, the molar absorptivity of the complex FeLn2+ will be determined by using the absorbance of the complex and its concentration. The absorbance will be found by using a spectrophotometer. For the next part of the lab, the formula of the complex will be determined by also using the volume of ligand and the absorbance again. Experimental There are two different parts to the experiement. In the first part, five 100 mL flasks of 5 mL ligand solution, 5 mL 2 M sodium acetate, 4 mL 3 M NH2OH, and 1-5 mL Fe2+ solution are diluted with water.
* Let the crystals dry for one week, record the weight and take a sample and put into a glass capillary tube to obtain a melting point using the Melt-Temp machine. * Since volatile inflammable substances (ether, ethanol) and heat are used in this procedure, be very careful while conducting this experiment. Name | Structure | Molar Mass | Trimyristin | | 723.16 g mol−1 | Weight before recrystallization | Weight after recrystallization | % Recovered | Literature Mp | Experimental Mp | % Error | 2.0grams | 0.09 grams
ENZYME worksheet 1. Label the diagram [pic] a. substrate b. active site c. enzyme d. products 2. Answer true of false to the following statements: ___T____ Enzymes interact with specific substrates ___F____ Enzymes change shape after a reaction occurs ___T____ Enzymes speed up reactions. ___F____ One enzyme can be used for many different types of chemical reactions. ___T____ Enzyme reactions can be slowed or halted using inhibitors.
I added varying levels of substrate to the test tubes in each experiment. The amount of substrates were .5 grams, 1 gram, 2 grams, 4 grams, and 8 grams. The output of the experiment (the dependent variable) was the number of molecules of product formed per minute at (106). RESULTS Test Tube # | pH Level | Amount of Substrate | Number of Molecules of Product Formed per Minute (106) | Test Tube #1 | 3 | .5g | 19 | Test Tube #2 | 3 | 1.0g | 39 | Test Tube #3 | 3 | 2.0g | 82 | Test Tube #4 | 3 | 4.0g | 96 | Test Tube #5 | 3 | 8.0g | 96 | | | | | Test Tube #1 | 5 | .5g | 39 | Test Tube #2 | 5 | 1.0g | 81 | Test Tube #3 | 5 | 2.0g | 168 | Test Tube #4 | 5 | 4.0g | 198 | Test Tube #5 | 5 | 8.0g | 198 | | | | | Test Tube #1 | 7 | .5g | 72 | Test Tube #2 | 7 | 1.0g | 145 | Test Tube #3 | 7 | 2.0g | 300 | Test Tube #4 | 7 | 4.0g |
Add 0.2 ml of yeast invertase and incubate at the same temperature for 6 mins. After incubation quench with 8.0 ml of 0.1M H2SO4 and mix thoroughly. Transfer 0.1ml aliquot of sample to a separate test tube and add 0.9 ml of H2O followed by 4ml of WSG and incubate at afore mentioned temperature for a period of 15 mins. The mixture is then assayed at 500nm. Procedure is to be repeated using temperature of 0o, 60o, and 80o C. Introduction Enzymes are single-chain or multiple chain proteins that act as biological catalysts with the inherent ability to promote specific chemical reactions in vivo, as well as in vitro.
Diffusion and Osmosis Lab Part 1A: Diffusion Evidence: Table 1: The Color of Different Solutions Before and After Diffusion for 30 Minutes Content | Initial Solution Color | Final Solution Color | Bag15% glucose1%starch | Cloudy white | Very dark purple | BeakerWater + IKI | Golden yellow | Golden yellow | The above table represents the final and initial colors of the solution that was inside of the dialysis tubing and the solution that was in the beaker. A 15% glucose and 1% starch solution was placed inside of the dialysis tubing. The initial color of the solution in the bag was a cloudy white but after 30 minutes of sitting in the beaker of water and IKI the color turned a very dark purple almost black. Inside of the beaker was a water and IKI solution. After sitting for 30minutes this solution stayed the same color.
Confirmation tests are then used to confirm the found identity of the cation or anion. Materials and Methods: Cation Tests- The elements, potassium, iron (III), zinc, copper(II), and cobalt were each labeled on five test tubes, each consisting ten drops of the labeled metal solution. The color was noted. For the metal hydroxide elimination test, 6 M NaOH was added dropwise to each test tube till a precipitate was observed or till 20 drops were reached. 10 drops of NaOH were added to each precipitate to test for amphoteric species.
After waiting a minute, 50 µL of pyruvate was then added to the cuvette and then mixed. The cuvette was then placed in a spectrophotometer and the initial absorbance of the solution was measured. Subsequent absorbance measurements were taken each minute over a three minute period. The overall enzyme activity was measured indirectly through the absorbance of the solution. Absorbance was measured using a spectrophotometer, an instrument that measures the light absorption of a solution at a given wavelength with a spectrophotoelectric cell.