Enzyme Lab Essay

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Enzyme Lab Conclusion Questions 3. At what temperature did catalase work best? -Catalase worked best at room temperature. 4. Explain what it means to denature an enzyme. -To denature an enzyme means to change its shape by breaking the hydrogen bonds that holds its shape, thus rendering it useless. An enzyme can be denatured with heat, or with a change in pH. 5. What factors did you need to control in your tests? -During these tests, temperature needed to be controlled. 6. If you’ve ever used hydrogen peroxide as an antiseptic to treat a cut or scrape, you know that it foams as soon as it touches and open wound. How can you account for this observation? -There are catalase enzymes in your body and they break down the hydrogen peroxide into water and oxygen. The foam is the oxygen bubbles that form in the water. 7. Why is it important for organisms to regulate internal temperatures or metabolic processes at different temperatures? -Certain vital enzymes will become inactive if the temperature is too cold, and denatured if the temperature is too hot. The organism cannot function without these vital enzymes. 8. Explain how the process of blanching is used in the food industry and why a specific temperature is required. -Blanching is used in the food industry to quickly heat products containing peroxidase to denature the proteins in an attempt to stop peroxidase from producing the foul-tasting hydrogen peroxide molecules. A specific temperature is required because the food needs to be heated, but not so much so that it gets cooked, or not so little so that the peroxidase remains

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