Enzymes Amylase: | Tracing the Breakdown of Starch | Rashaud Pickering, Ashley Bagnis, Stephanie Alvarado, Elbany Angulo 3614098 Section U-14 | Signature___________________ Abstract An experiment was conducted to find out what the optimal temperature for amylase to break down starch was. An Iodine test was used to find evidence of starch formation. Three drops of iodine were dropped into two sets of spot plates. There were four test tubes that contained human amylase and four test tubes that contained fungal amylase. These specimens were all tested before being placed into their respective ice or water baths.
* Secondly you have to add five drops of phenolphthalein the test tube which you are using. * Then measure out 5 cm of milk using a syringe or a measuring cylinder, and then add this to the test tube. * After this measure out 7 cm of sodium carbonate solution using a different measuring cylinder or a syringe and then add this to the test tube, which will make the solution turn pink. * Next you will have to place a thermometer in the test tube, but you will have to be careful as the experiment could fall over. * Then you will have to put the test tube in a water bath and leave it until the contents reach the same temperature as the water bath.
After heating, the mixture was cooled to room temperature and filtered by vacuum filtration into a fritted funnel to yield a purple product. The product was washed 3 times with (5mL) portions of chilled 6M HCL, then Ethanol, and lastly with acetone. The resulting product was placed into a vial and left to dry in a vacuum desiccator for 1 week and weighed the next week. The yield was 6.029g. The second experiment, procedure 1, combined [Co(NH3)5 (H2O)]Cl2 (0.0060M, 1.52g) and (25mL) of distilled water to an 125mL Erlenmeyer flask.
The third test will utilize thin layer chromatography to evaluate the purity of the aspirin as well as testing for the presence of leftover salicylic acid or other by products of the reactions. Experimental: Week 1: For the synthesis of the aspirin, 250 mL of water was boiled. 1.5 g. of salicylic acid were poured on a test tube. Then, 3.5 mL of acetic anhydride and four drops of 85% phosphoric acid were added. A cotton ball was placed to prevent vapor escape.
Liver Enzyme Lab Purpose: The purpose of this experiment is to determine the optimal temperature of Catalase. Procedure: Place a small amount of blended liver a baggie Heat water on a hotplate (or use ice if needed) to bring your water bath to your assigned temperature (measure with thermometer) Place baggie with liver into water, brining both to desired temperature. When ready, add 2 filter paper discs to the liver in the baggie and let it sit in the water for 3-4 minutes. Meanwhile, set up your apparatus, as follows, to measure O2 production (volume in mL) *Perform TWO trials When ready, mix the peroxide with the discs, measure the volume of the gas produced. Materials: -Thermometer -Filter paper discs -Inverted graduated cylinder -Hydrogen peroxide -Cafeteria tray -Rubber Stopper with tube -Gas trapping bottle -Blender -Liver -Ziploc bag -Hotplate -Beakers Temperature (°C) Trial 1 (mL) Trial 2 (mL) Average (mL) 0 120 140 130 10 118 115 116.5 20 118 123 120.5 30 110 115 112.5 40 171 159 165 50 102 92 97 60 0 100 50 70 20 0 10 80 0 0 0 90 0.5 12 6.25 100 0 0 0 ￼ Analysis: Looking at the graph above, we notice overall the graph increases to a peak, and then begins to decrease all the way down to zero.
We observed different solutes (NaCl, Ovalbumin, Glucose, Sucrose, and Water) in the dialysis tubing. The problem was what environment the solutes would create and whether water would diffuse in or out of the cell. I predicted that all of the tubes will be in a hypotonic environment after the 30 minutes because there is
In our 2nd, we examined the effect of warmer temperature. Our research question was, ‘does catalase denature in stages or all at once as temperature increases?’ Our hypothesis was: if temperature increases, then the catalase will denature in stages as the temperature increase, the catalase will slowly stop working. We followed the procedures in the lab manual (choinski and karatit 2014) with the following expetions: For experiment 1, we used 1.5% hydrogen peroxide concentration and experimented this concentration at 4*c, 24*c (room temp. ), 44*c, 52*c and 60*c. For experiment 2, we used 3% hydrogen peroxide concentration and experiment this concentration at 21*c (room temp. ), 35*c, 45*c, 55*c, & 60*c. Figure 1: Effect of temperature on catalase activity.
ii. Materials & Methods: First, testing the enzyme specificity. Obtain three test tubes and mark them at the 1 cm and 5 cm levels. First tube: fill it to the first mark with catalase buffered at pH 7.0, the optimum pH for catalase. Then fill the second mark with hydrogen peroxide.
By using TLC and IR spectrum, we will see the difference between benzophenone (starting material) and diphenylmethanol (final product). Experimental Procedure: Benzophenone (358mg, 2.0mmol) was dissolved in ethanol (5ml) in a 25ml round bottomed flask. The solid did not completely dissolve, so the flask was swirled with magnetic stirrer bar. In a small test tube, sodium borohydride (87mg, 2.2mmol) was dissolved in cold water (1.5ml). This solution was added drop wise to the stirred ethanolic solution of benzophenone at room temperature.
By doing this, it will show the initial pH before any acid or base has been added into the solution. Followed by that, add 5 drops of .1 M HCl into the tube, mix, and record the new pH in the acid row found on Table 1. After that continue adding 5 drops at a time until 30 drops total have been added, make sure to record the pH in-between every 5 drops. Once 30 drops have been reached, quit adding and record the final pH. Using the other test tube of the same solution, again, measure the initial pH and repeat the same procedure except adding .1 M NaOH, followed by recording the data in the base row in