Enzyme Concentration On The Rate Of Reaction

787 Words4 Pages
INTRODUCTION: I am trying to investigate enzyme concentration and the rate of reaction. In this investigation, a proteolytic enzyme is used to catalyze the breakdown of protein into peptides. Protein gelatin is the substrate used. As the reaction progress, the gelatin is broken down and become less viscous. This has effect on the time it takes for the gelatin to go through the funnel in to the beaker. HYPOTHESIS: I predicted the higher the enzyme concentration, the less time it will take for the gelatin to pass through the funnel because the more the proteolytic enzyme concentration, the faster the breakdown of the protein (gelatin) into peptides. EQUIPMENTS/APPARATUS: Pipette, test tube, trypsin, gelatin (protein), beaker, glass rod, clamp stand, funnel, stop clock and ph buffer. PROCEDURE: I used the pipette to measure 3 cm of pH buffer into a test tube. The test tube was handled with care to prevent it from breaking. I then used another pipette to measure 7cm of 0% trypsin solution into the same test tube containing the pH buffer. 75 gram of gelatin was weighed into a beaker. I then poured the mixture of pH buffer and the trypsin solution in the beaker containing 75 gram of gelatine. Using the glass rod, I carefully and gently stir the mixture for approximately 15 seconds, using the stop clock. Using a clamp stand, I suspended the funnel over an empty beaker. I pour all the mixture into the funnel after leaving it for 5 minutes and again started the stop clock. I recorded all the time taken for the gelatin to pass through the funnel. I repeated the procedure for 1%, 3% and 5% of enzyme concentration VARIABLES: INDEPENDENT VARIABLE: Enzyme concentration (trypsin) was the independent variable because they are the changes that were directly caused by me. DEPENDENT VARIABLE: Rate of reaction (1/T) that is the time taken for the

More about Enzyme Concentration On The Rate Of Reaction

Open Document