Enzyme Activity Essay

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Investigation of the effect of metal ions on enzyme activity Objective: The effect of lead (II) ion and silver ion on the enzyme activity of invertase Introduction: Hypothesis: The activity of the invertase will be inhibited by the heavy metal ions Prediction made from the hypothesis: Biological principle: Heavy metal ions are non-competitive inhibitors. They do not compete with the substrates for the active sites of enzymes. They attach to other parts of the enzyme molecules and cause changes in the structure of the active site. The substrate molecules can no longer bind to the active sites and the rate of enzymatic reaction decreases. Therefore, they prevent the enzyme reactions from taking place. Independent Variable: Presence or absence of metal ions in the solution There are two solutions with two different metal ions and two are replaced by distilled water without metal ions Dependent Variable: The effect of metal ions on the enzymes activity of intervase It can be measured via Benedict’s test by the colour change and the quantity of the precipitate formed Controlled Variable: The amount of the invertase in each solution The time of the reaction take place Control set-up: Replace the metal ions in the solution by the same volume of distilled water Assumptions: All reducing sugar has reacted with B solution, invertase works well in the given pH and temperature Materials and apparatus: * 10mM lead (II) nitrate * 10mM silver nitrate * 50g/L sucrose solution * 0.03g/L invertase solution * Benedict’s solution * 6 test tubes * Measuring cylinder * 1 beaker * Bunsen Burner * Tripod * Wire gauze Procedures: Silver nitrate: 1. 0.5mL of silver nitrate solution is added to 1mL of sucrose solution. 2. 1.5mL of invertase solution is added to the mixture. 3. The mixture is

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