Enzyme Activity Essay

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Internal Assessment I Aim: Investigate a factor affecting the rate of enzyme activity. Research Question: What is the effect of the different concentrations (0.6%, 1.2%, 1.8%, 2.4%, 3.0%) of the substrate hydrogen peroxide (H2O2) towards the rate of reaction with the enzyme catalase found in yeast? Variables: | Units, Range, Uncertainty: | Measured using: | Independent Variables | Different concentration of hydrogen peroxide (0.6%, 1.2%, 1.8%, 2.4%, 3.0%) | A volumetric flask and measuring cylinder (to dilute the 3.0% hydrogen peroxide) | Dependent Variables | Rate of reaction (cm3s-1) | A measuring cylinder and stop watch (to measure the amount of foam produced against a specified time, thus finding the rate of reaction) | Hypothesis: Prediction, based on observations: | Annotated sketch graph to show predicted outcomes: | If the rate of activity of an enzyme catalase is related to the concentration of the substrate, then with an increased concentration of H2O2, I predict that there will also be an increase in the volume of foam produced by the reaction between the catalase enzyme found in the yeast and the hydrogen peroxide, signifying the increase in the rate of enzyme activity. The least amount of foam will be produced when yeast is reacted with hydrogen peroxide solution of the lower concentration (in this experiment’s case, lowest being 0.6%), signifying the lower rate of reaction when the substrate concentration is lower. On the other hand, the most amount of foam will be produced when yeast is reacted with the hydrogen peroxide with a higher concentration (in this experiment’s case, highest being 3.0%), signifying a faster rate of reaction when the substrate concentration is higher. With this, it is predicted that as the concentration of the substrate increases, so does the rate of reaction. | | Scientific explanation for

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